{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T07:05:59Z","timestamp":1793516759228,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Isolates of\n            <jats:italic>Vibrio harveyi<\/jats:italic>\n            , a prawn pathogen, have demonstrated multiple antibiotic resistance to commonly used antimicrobial agents, such as oxytetracycline. In this paper, we describe the cloning and characterization of two tetracycline resistance determinants from\n            <jats:italic>V. harveyi<\/jats:italic>\n            strain M3.4L. The first resistance determinant, cloned as a 4,590-bp fragment, was identical to\n            <jats:italic>tetA<\/jats:italic>\n            and flanking sequences encoded on transposon Tn\n            <jats:italic>10<\/jats:italic>\n            from\n            <jats:italic>Shigella flexneri.<\/jats:italic>\n            The second determinant, cloned as a 3,358-bp fragment in pATJ1, contains two open reading frames, designated\n            <jats:italic>tet35<\/jats:italic>\n            and\n            <jats:italic>txr<\/jats:italic>\n            .\n            <jats:italic>tet35<\/jats:italic>\n            encodes a 369-amino-acid protein that was predicted to have nine transmembrane regions. It is a novel protein which has no homology to any other drug resistance protein but has low levels of homology (28%) to Na\n            <jats:sup>+<\/jats:sup>\n            \/H\n            <jats:sup>+<\/jats:sup>\n            antiporters. Transposon mutagenesis showed that\n            <jats:italic>tet35<\/jats:italic>\n            and\n            <jats:italic>txr<\/jats:italic>\n            were required for tetracycline resistance in a heterologous\n            <jats:italic>Escherichia coli<\/jats:italic>\n            host. Tetracycline accumulation studies indicate that\n            <jats:italic>E. coli<\/jats:italic>\n            carrying\n            <jats:italic>tet35<\/jats:italic>\n            and\n            <jats:italic>txr<\/jats:italic>\n            can function as an energy-dependent tetracycline efflux pump but is less efficient than TetA.\n          <\/jats:p>","DOI":"10.1128\/aac.46.4.1038-1045.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1038-1045","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Genetic Determinants of Tetracycline Resistance in\n            <i>Vibrio harveyi<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Jeanette W. P.","family":"Teo","sequence":"first","affiliation":[{"name":"Programme in Environmental Microbiology, Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa M. 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