{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:21:31Z","timestamp":1793157691178,"version":"4.5.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The \u03b2-lactamase gene\n            <jats:italic>blaA<\/jats:italic>\n            <jats:sub>BPS<\/jats:sub>\n            in\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            was cloned and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A \u03b2-lactamases and a relationship to the PenA (in\n            <jats:italic>B. cepacia<\/jats:italic>\n            ) and BlaI (in\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            ) lineages.\n          <\/jats:p>","DOI":"10.1128\/aac.46.4.1132-1135.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1132-1135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Cloning and Expression of Class A \u03b2-Lactamase Gene\n            <i>blaA<\/i>\n            <sub>BPS<\/sub>\n            in\n            <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Terence K. M.","family":"Cheung","sequence":"first","affiliation":[{"name":"Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. L.","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C. Y.","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Y.","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Y.","family":"Chau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.1.6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/153.1.167"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.17.3.355"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.313"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.1.1"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/10.1.1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3461561"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00282468"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.2.468"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2034-2038.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(84)90060-9"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2399"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(99)00165-X"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.4.1132-1135.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.4.1132-1135.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:27:47Z","timestamp":1645464467000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.46.4.1132-1135.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1128\/AAC.46.4.1132-1135.2002"],"URL":"https:\/\/doi.org\/10.1128\/aac.46.4.1132-1135.2002","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]},"assertion":[{"value":"2001-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}