{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:42:32Z","timestamp":1773283352054,"version":"3.50.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2002,8,1]],"date-time":"2002-08-01T00:00:00Z","timestamp":1028160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We screened 615 gram-positive isolates from 150 healthy children for the presence of the\n            <jats:italic>erm<\/jats:italic>\n            (A),\n            <jats:italic>erm<\/jats:italic>\n            (B),\n            <jats:italic>erm<\/jats:italic>\n            (C),\n            <jats:italic>erm<\/jats:italic>\n            (F), and\n            <jats:italic>mef<\/jats:italic>\n            (A) genes. The\n            <jats:italic>mef<\/jats:italic>\n            (A) genes were found in 20 (9%) of the macrolide-resistant isolates, including\n            <jats:italic>Enterococcus<\/jats:italic>\n            spp.,\n            <jats:italic>Staphylococcus<\/jats:italic>\n            spp., and\n            <jats:italic>Streptococcus<\/jats:italic>\n            spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn\n            <jats:italic>1207.1<\/jats:italic>\n            , a genetic element carrying\n            <jats:italic>mef<\/jats:italic>\n            (A) recently described in\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . The three\n            <jats:italic>Staphylococcus<\/jats:italic>\n            spp. did not carry\n            <jats:italic>orf1<\/jats:italic>\n            to\n            <jats:italic>orf3<\/jats:italic>\n            . A gram-negative\n            <jats:italic>Acinetobacter junii<\/jats:italic>\n            isolate also carried the other seven ORFs described in Tn\n            <jats:italic>1207.1.<\/jats:italic>\n            A\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolate, a\n            <jats:italic>Streptococcus intermedius<\/jats:italic>\n            isolate, a\n            <jats:italic>Streptococcus<\/jats:italic>\n            sp. isolate, and an\n            <jats:italic>Enterococcus<\/jats:italic>\n            sp. isolate had their\n            <jats:italic>mef<\/jats:italic>\n            (A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the\n            <jats:italic>mef<\/jats:italic>\n            (A) gene from\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.46.8.2513-2517.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:07:23Z","timestamp":1027897643000},"page":"2513-2517","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Distribution of\n            <i>mef<\/i>\n            (A) in Gram-Positive Bacteria from Healthy Portuguese Children"],"prefix":"10.1128","volume":"46","author":[{"given":"Vicki A.","family":"Luna","sequence":"first","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Heiken","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Judge","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Ulep","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Van Kirk","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique","family":"Luis","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of Lisbon, Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Bernardo","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of Lisbon, Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Leitao","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of Lisbon, Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn C.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.1.5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1093\/jac\/44.3.329","volume":"44","year":"1999","unstructured":"Chung, W. O., K. Young, Z. Leng, and M. C. Roberts. 1999. Mobile elements carrying ermF and tetQ genes in gram-positive and gram-negative bacteria. Antimicrob. Agents Chemother.44:329-335.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/321001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1493"},{"key":"e_1_3_2_6_2","first-page":"19","volume":"44","year":"1999","unstructured":"Luna, V. A., P. Coates, E. A. Eady, J. H. Cove, T. T. H. Nguyen, and M. C. Roberts. 1999. A variety of gram-positive bacteria carry mobile mef genes. J. Antimicrob. Chemother.44:19-25.","journal-title":"J. Antimicrob. 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Detection of erythromycin resistant methylase gene by the polymerase chain reaction. Mol. Cell. Probes11:317-322.","journal-title":"Mol. Cell. Probes"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090040801"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.6.1367"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.12.2823"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/jac\/48.2.317","volume":"48","year":"2001","unstructured":"Rudolph, K. M., A. J. Parkinson, and M. C. Roberts. 2001. Mechanisms of erythromycin and trimethoprim resistance in the Alaskan Streptococcus pneumoniae 6B clone. J. Antimicrob. Chemother.48:317-319.","journal-title":"J. Antimicrob. 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