{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:46:37Z","timestamp":1792457197466,"version":"4.5.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Chryseobacterium gleum<\/jats:italic>\n            (previously included in the\n            <jats:italic>Flavobacterium<\/jats:italic>\n            IIb species) is a gram-negative aerobe that is a source of nosocomial infections. An Ambler class B \u03b2-lactamase gene was cloned and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            from reference strain\n            <jats:italic>C. gleum<\/jats:italic>\n            CIP 103039 that had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. The purified \u03b2-lactamase, CGB-1, with a pI value of 8.6 and a determined relative molecular mass of ca. 26 kDa, hydrolyzed penicillins; narrow- and expanded-spectrum cephalosporins; and carbapenems. CGB-1 was a novel member of the molecular subclass B1 of metallo-enzymes. It had 83 and 42% amino acid identity with IND-1 from\n            <jats:italic>Chryseobacterium indologenes<\/jats:italic>\n            and BlaB from\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            , respectively. Thus, in addition to the previously characterized clavulanic acid-inhibited extended-spectrum \u03b2-lactamase CGA-1 of Ambler class A,\n            <jats:italic>C. gleum<\/jats:italic>\n            produces a very likely chromosome-borne class B \u03b2-lactamase.\n          <\/jats:p>","DOI":"10.1128\/aac.46.9.2791-2796.2002","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T19:36:19Z","timestamp":1033500979000},"page":"2791-2796","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Genetic and Biochemical Characterization of CGB-1, an Ambler Class B Carbapenem-Hydrolyzing \u03b2-Lactamase from\n            <i>Chryseobacterium gleum<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Samuel","family":"Bellais","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_3_2","first-page":"127","volume":"171","year":"1999","unstructured":"Bellais, S., S. L\u00e9otard, L. Poirel, T. Naas, and P. Nordmann. 1999. Molecular characterization of a carbapenem-hydrolyzing \u03b2-lactamase from Chryseobacterium (Flavobacterium) indologenes. FEMS Microbiol. Lett.171:127-132.","journal-title":"FEMS Microbiol. 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