{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:49:10Z","timestamp":1791103750600,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Fifty-six azithromycin-resistant (MICs, 2.0 to 4.0 \u03bcg\/ml)\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            strains with cross-resistance to erythromycin (MICs, 2.0 to 64.0 \u03bcg\/ml), isolated in Canada between 1997 and 1999, were characterized, and their mechanisms of azithromycin resistance were determined. Most (58.9%) of them belonged to auxotype-serotype class NR\/IB-03, with a 2.6-mDa plasmid. Based on resistance to crystal violet (MICs \u2265 1 \u03bcg\/ml), 96.4% of these macrolide-resistant strains appeared to have increased efflux. Nine of the eleven strains selected for further characterization were found to have a promoter region\n            <jats:italic>mtrR<\/jats:italic>\n            mutation, a single-base-pair (A) deletion in the 13-bp inverted repeat, which is believed to cause overexpression of the\n            <jats:italic>mtrCDE<\/jats:italic>\n            -encoded efflux pump. The two remaining macrolide-resistant strains (erythromycin MIC, 64.0 \u03bcg\/ml; azithromycin MIC, 4.0 \u03bcg\/ml), which did not have the mutation in the\n            <jats:italic>mtrR<\/jats:italic>\n            promoter region, were found to have a C2611T mutation (\n            <jats:italic>Escherichia coli<\/jats:italic>\n            numbering) in the peptidyltransferase loop in domain V of the 23S rRNA alleles. Although mutations in domain V of 23S rRNA alleles had been reported in other bacteria, including\n            <jats:italic>E. coli<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            , and\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            , this is the first observation of these mutations associated with macrolide resistance in\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.46.9.3020-3025.2002","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T19:36:19Z","timestamp":1033500979000},"page":"3020-3025","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":158,"title":["Mutation in 23S rRNA Associated with Macrolide Resistance in\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Lai-King","family":"Ng","sequence":"first","affiliation":[{"name":"National Laboratory for Sexually Transmitted Diseases, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Martin","sequence":"additional","affiliation":[{"name":"National Laboratory for Sexually Transmitted Diseases, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Liu","sequence":"additional","affiliation":[{"name":"National Laboratory for Sexually Transmitted Diseases, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Bryden","sequence":"additional","affiliation":[{"name":"National Laboratory for Sexually Transmitted Diseases, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.319-323.2001"},{"key":"e_1_3_2_4_2","unstructured":"Dillon J. 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