{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:54:45Z","timestamp":1781625285114,"version":"3.54.5"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            NEL-1 was isolated from a urine sample of a patient hospitalized in a long-term care facility. Strain NEL-1 produced a \u03b2-lactamase with a pI of 5.2 conferring resistance to amoxicillin and amoxicillin-clavulanic acid. Sequencing of a PCR amplicon by using TEM-specific primers revealed a novel\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            gene,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-67<\/jats:sub>\n            . TEM-67 was an IRT-1-like TEM derivative related to TEM-65 (Lys39, Cys244) with an additional Leu21Ile amino acid substitution in the leader peptide. The biochemical properties of TEM-67 were equivalent to those described for TEM-65. Analysis of sequences surrounding\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-67<\/jats:sub>\n            revealed that it was located on a transposon, Tn\n            <jats:italic>1<\/jats:italic>\n            , which itself was located on a 48-kb non-self-transferable plasmid, pANG-1. Electroporation of plasmid pANG-1 into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            DH10B resulted in the integration of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-67<\/jats:sub>\n            into the chromosome, whereas it remained episomal in the\n            <jats:italic>P. mirabilis<\/jats:italic>\n            CIP103181 reference strain. Further characterization of pANG-1 revealed the presence of two identical sequences on both sides of Tn\n            <jats:italic>1<\/jats:italic>\n            that contained an IS\n            <jats:italic>26<\/jats:italic>\n            insertion sequence followed by a novel colicin gene,\n            <jats:italic>colZ<\/jats:italic>\n            , which had 20% amino acid identity with other colicin genes. The characterization of this novel TEM derivative provides further evidence for the large diversity of plasmid-encoded \u03b2-lactamases produced in\n            <jats:italic>P. mirabilis<\/jats:italic>\n            and for their spread to other enterobacterial species through transposable-element-mediated events.\n          <\/jats:p>","DOI":"10.1128\/aac.47.1.19-26.2003","type":"journal-article","created":{"date-parts":[[2002,12,24]],"date-time":"2002-12-24T04:39:08Z","timestamp":1040704748000},"page":"19-26","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Integration of a Transposon Tn\n            <i>1<\/i>\n            -Encoded Inhibitor-Resistant \u03b2-Lactamase Gene,\n            <i>bla<\/i>\n            <sub>TEM-67<\/sub>\n            from\n            <i>Proteus mirabilis<\/i>\n            , into the\n            <i>Escherichia coli<\/i>\n            Chromosome"],"prefix":"10.1128","volume":"47","author":[{"given":"Thierry","family":"Naas","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Zerbib","sequence":"additional","affiliation":[{"name":"Unite\u0301 d'Hygie\u0300ne, Ho\u0302pital Foch, 92151 Suresnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.11.2671"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.2.183"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.4.447"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1930-1935.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.2.913-916.1987"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.2.209"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.8.1881"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.4.537"},{"key":"e_1_3_2_11_2","unstructured":"Galas D. J. and M. Chandler. 1989. Bacterial insertion sequences p. 109-162. In D. E. Berg and M. M. Howe (ed.) Mobile DNA. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1128\/AAC.44.2.467-469.2000","volume":"44","year":"2000","unstructured":"Girlich, D., A. Karim, L. Poirel, M. H. Cavin, C. Verny, and P. Nordmann. 2000. Molecular epidemiology of an outbreak due to IRT-2 \u03b2-lactamase-producing strains of Klebsiella pneumoniae in a geriatric department. J. Antimicrob. Chemother.44:467-473.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.367"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00328705"},{"key":"e_1_3_2_15_2","unstructured":"Jacoby G. A. 1986. Resistance of plasmids of Pseudomonas p. 265-294. In J. R. Sokatch (ed.) Bacteria: a treatise on structure and function vol. X. Academic Press Inc. San Diego Calif."},{"key":"e_1_3_2_16_2","first-page":"624","volume":"10","year":"2001","unstructured":"Kleanthous, C., and D. Walker. 2001. Immunity proteins: enzyme inhibitors that avoid the active site. Trends Biochem. Sci.10:624-631.","journal-title":"Trends Biochem. Sci."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.10.2709-2714.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.11.2580"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286188"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.725-774.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/ageing\/22.3.221","volume":"22","year":"1993","unstructured":"Michielsen, W., D., G. Vandevondele, G. Verschraegen, G. Clayes, and M. Afschrift. 1993. Bacterial surveillance cultures in a geriatric ward. Age Ageing22:221-226.","journal-title":"Age Ageing"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.48.3.199-221.1984"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.18.6319"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0022-2836(83)80148-X","volume":"170","year":"1983","unstructured":"Morlon, J., R. Lloubes, S. Varenne, M. Chartier, and C. Lazdunski. 1983. Complete nucleotide sequence of the structural gene for colicin A, a gene translated at non-uniform rate. J. Mol. Biol.170:271-285.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/136.3.721"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1281"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.1.235-249.2001"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6721-6725.2001"},{"key":"e_1_3_2_29_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A3. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_30_2","first-page":"S74","volume":"3","year":"1997","unstructured":"Nicolas-Chanoine, M. H., H. Chardon, J. L. Avril, Y. Cattoen, J. C. Croix, H. Dabernat, et al. 1997. Susceptibility of Enterobacteriaceae to betalactams and fluoroquinolones: a French multicentre study. Clin. Microbiol. Infect.3:S74-S75.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/BF00268276","volume":"172","year":"1979","unstructured":"Oka, A., N. Nomura, M. Morita, H. Sugisaki, K. Sugimoto, and M. Takanami. 1979. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity Mol. Gen. Genet.172:151-159.","journal-title":"Gen. Genet."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0002-9343(96)90014-5","volume":"100","year":"1996","unstructured":"Orr, P. H., L. E. Nicolle, H. Duckworth, J. Brunka, J. Kennedy, D. Murray, and G. K. Harding. 1996. Febrile urinary infection in the institutionalized elderly. Am. J. Med.100:71-77.","journal-title":"Am. J. Med."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.8.1131"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2188"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.23.6973-6977.1995"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1128\/AAC.44.3.622-632.2000","volume":"44","year":"2000","unstructured":"Poirel, L., I. Le Thomas, T. Naas, A. Karim, and P. Nordmann. 2000. Biochemical sequence analyses of GES-1, a novel class A extended-spectrum \u03b2-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob. Agents and Chemother.44:622-632.","journal-title":"Agents and Chemother."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90149-1"},{"key":"e_1_3_2_38_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.7.3350-3357.1987"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.15.4919-4928.1997"},{"key":"e_1_3_2_41_2","first-page":"307","volume":"9","year":"1959","unstructured":"Steers, E., E. I. Foltz, B. S. Graves, and J. Riden. 1959. An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiot. Chemother.9:307-311.","journal-title":"Antibiot. Chemother."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi990758z"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2000.tb00541.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.1.19-26.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.1.19-26.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:59:45Z","timestamp":1645466385000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.47.1.19-26.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1128\/AAC.47.1.19-26.2003"],"URL":"https:\/\/doi.org\/10.1128\/aac.47.1.19-26.2003","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]},"assertion":[{"value":"2002-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}