{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:48:32Z","timestamp":1791103712044,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Seventy-six\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            isolates, isolated between 1940 and\n1987, and seven\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            isolates, isolated\nbetween 1963 and 1987, were screened for the presence of acquired\n            <jats:italic>mef<\/jats:italic>\n            (A),\n            <jats:italic>erm<\/jats:italic>\n            (B),\n            <jats:italic>erm<\/jats:italic>\n            (C), and\n            <jats:italic>erm<\/jats:italic>\n            (F)\ngenes by using DNA-DNA hybridization, PCR analysis, and sequencing. The\n            <jats:italic>mef<\/jats:italic>\n            (A),\n            <jats:italic>erm<\/jats:italic>\n            (B), and\n            <jats:italic>erm<\/jats:italic>\n            (F) genes were all\nidentified in a 1955\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolate, while the\n            <jats:italic>erm<\/jats:italic>\n            (C) gene was identified in a 1963\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolate. Similarly, both the\n            <jats:italic>mef<\/jats:italic>\n            (A) and\n            <jats:italic>erm<\/jats:italic>\n            (F) genes\nwere identified in a 1963\n            <jats:italic>N. meningitidis<\/jats:italic>\n            isolate. All four\nacquired genes were found in later isolates of both species. The\n            <jats:italic>mef<\/jats:italic>\n            (A) gene from a 1975\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolate was\nsequenced and had 100% DNA and amino acid identity with the\n            <jats:italic>mef<\/jats:italic>\n            (A) gene from a 1990s\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            isolate. Selected early isolates were able to transfer their acquired\ngenes to an\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            recipient, suggesting that\nthese genes are associated with conjugative transposons. These isolates\nare the oldest of any species to carry the\n            <jats:italic>mef<\/jats:italic>\n            (A) gene and\namong the oldest to carry these\n            <jats:italic>erm<\/jats:italic>\n            genes.\n          <\/jats:p>","DOI":"10.1128\/aac.47.12.3877-3880.2003","type":"journal-article","created":{"date-parts":[[2003,11,24]],"date-time":"2003-11-24T22:53:35Z","timestamp":1069714415000},"page":"3877-3880","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Acquired Macrolide Resistance Genes in Pathogenic\n            <i>Neisseria<\/i>\n            spp. Isolated between 1940 and1987"],"prefix":"10.1128","volume":"47","author":[{"suffix":"Jr.","given":"Sydney","family":"Cousin","sequence":"first","affiliation":[{"name":"Departments\rof Pathobiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L. H.","family":"Whittington","sequence":"additional","affiliation":[{"name":"Medicine,University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn C.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Departments\rof Pathobiology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.9.2335"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.7.1598"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.1.5"},{"key":"e_1_3_2_5_2","unstructured":"Morb. Mortal. Wkly. 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Birkhauser Verlag Basel Switzerland.","DOI":"10.1007\/978-3-0348-8105-0_2"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(03)00039-6"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2503-2506.2000"},{"key":"e_1_3_2_16_2","first-page":"19","volume":"44","year":"1999","unstructured":"Luna, V. A., P. Coates, J. H. Cove, A. E. Eady, T. T. H. Nguyen, and M. C. Roberts. 1999 . A variety of gram-positive bacteria carry mobile mef genes. J. Antimicrob. Chemother.44:19-25.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.4.1575-1580.2000"},{"key":"e_1_3_2_18_2","unstructured":"National\nCommittee for Clinical Laboratory Standards. 1995.\nApproved standard. Methods for dilution antimicrobial susceptibility\ntests for bacteria that grow aerobically. M7-A3. 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