{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:05:25Z","timestamp":1791273925261,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We\ninvestigated a\n            <jats:italic>Klebsiella oxytoca<\/jats:italic>\n            isolate demonstrating\nresistance to imipenem, meropenem, extended-spectrum cephalosporins,\nand aztreonam. The MICs of both imipenem and meropenem were 32\u03bc\ng\/ml. The \u03b2-lactamase activity against imipenem and\nmeropenem was inhibited in the presence of clavulanic acid. Isoelectric\nfocusing studies demonstrated five \u03b2-lactamases with pIs of 8.2\n(SHV-46), 6.7 (KPC-2), 6.5 (unknown), 6.4 (probable OXY-2), and 5.4\n(TEM-1). The presence of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            genes was confirmed by specific PCR assays\nand DNA sequence analysis. Transformation and conjugation studies with\n            <jats:italic>Escherichia coli<\/jats:italic>\n            showed that the \u03b2-lactamase with a pI\nof 6.7,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            carbapenemase-2 (KPC-2), was\nencoded on an approximately 70-kb conjugative plasmid that also carried\nSHV-46, TEM-1, and the \u03b2-lactamase with a pI of 6.5. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            determinant was cloned in\n            <jats:italic>E. coli<\/jats:italic>\n            and conferred resistance to imipenem, meropenem, extended-spectrum\ncephalosporins, and aztreonam. The amino acid sequence of KPC-2 showed\na single amino acid difference, S174G, when compared with KPC-1,\nanother carbapenem-hydrolyzing \u03b2-lactamase from\n            <jats:italic>K.\npneumoniae<\/jats:italic>\n            1534. Hydrolysis studies showed that purified KPC-2\nhydrolyzed not only carbapenems but also penicillins, cephalosporins,\nand aztreonam. KPC-2 had the highest affinity for meropenem. The\nkinetic studies revealed that KPC-2 was inhibited by clavulanic acid\nand tazobactam. An examination of the outer membrane proteins of the\nparent\n            <jats:italic>K. oxytoca<\/jats:italic>\n            strain demonstrated that it expressed\ndetectable levels of OmpK36 (the homolog of OmpC) and a\nhigher-molecular-weight OmpK35 (the homolog of OmpF). Thus, carbapenem\nresistance in\n            <jats:italic>K. oxytoca<\/jats:italic>\n            3127 is due to production of the Bush\ngroup 2f, class A, carbapenem-hydrolyzing \u03b2-lactamase KPC-2.\nThis \u03b2-lactamase is likely located on a transposon that is part\nof a conjugative plasmid and thus has a very high potential for\ndissemination.\n          <\/jats:p>","DOI":"10.1128\/aac.47.12.3881-3889.2003","type":"journal-article","created":{"date-parts":[[2003,11,24]],"date-time":"2003-11-24T22:53:35Z","timestamp":1069714415000},"page":"3881-3889","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":163,"title":["Carbapenem-Resistant Strain of\n            <i>Klebsiellaoxytoca<\/i>\n            Harboring Carbapenem-Hydrolyzing\u03b2-LactamaseKPC-2"],"prefix":"10.1128","volume":"47","author":[{"given":"Hesna","family":"Yigit","sequence":"first","affiliation":[{"name":"Bristol-Myers\rSquibb Pharmaceutical Research Institute, Wallingford, Connecticut\r06492"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Marie","family":"Queenan","sequence":"additional","affiliation":[{"name":"The R. W. Johnson\rPharmaceutical Research Institute, Raritan, New Jersey\r08869"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Kamile","family":"Rasheed","sequence":"additional","affiliation":[{"name":"Division of Healthcare\rQuality Promotion, National Center for Infectious Diseases,\rCenters for Disease Control and Prevention, Atlanta, Georgia\r30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Biddle","sequence":"additional","affiliation":[{"name":"Division of Healthcare\rQuality Promotion, National Center for Infectious Diseases,\rCenters for Disease Control and Prevention, Atlanta, Georgia\r30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Domenech-Sanchez","sequence":"additional","affiliation":[{"name":"Unidad de Investigacion,\rHospital Son Dureta, Andrea Doria, Palma de Mallorca\r07014"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Alberti","sequence":"additional","affiliation":[{"name":"Area de Microbiologia,\rUniversidad de las Islas Baleares, Crtra.Valldemosa, Palma de Mallorca 07071, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Bush","sequence":"additional","affiliation":[{"name":"The R. W. Johnson\rPharmaceutical Research Institute, Raritan, New Jersey\r08869"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred C.","family":"Tenover","sequence":"additional","affiliation":[{"name":"Division of Healthcare\rQuality Promotion, National Center for Infectious Diseases,\rCenters for Disease Control and Prevention, Atlanta, Georgia\r30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.2.211"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.7.1636"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.179-184.1993"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.563"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_7_2","unstructured":"Chandler M. and J. Mahillon. 2002. Insertion sequences\nrevisited p. 328-330. In N. 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