{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:44:04Z","timestamp":1791938644754,"version":"4.3.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A 45-kb R plasmid, pRAS1, that confers resistance to tetracyclines, trimethoprim, and sulfonamides was isolated in 1989 from an atypical strain of the fish pathogen\n            <jats:italic>Aeromonas salmonicida<\/jats:italic>\n            . This plasmid could be transferred by conjugation to\n            <jats:italic>Escherichia coli<\/jats:italic>\n            with a high degree of efficiency (frequency, 0.48). The following year pRAS1 was isolated from\n            <jats:italic>A. salmonicida<\/jats:italic>\n            subsp.\n            <jats:italic>salmonicida<\/jats:italic>\n            in the same area. Incompatibility group U plasmid pRAS1 contained a drug resistance-determining region of 12 kb consisting of a class 1 integron similar to In4 of Tn\n            <jats:italic>1696<\/jats:italic>\n            but with a\n            <jats:italic>dfrA16<\/jats:italic>\n            gene cassette inserted. Close to IS\n            <jats:italic>6100<\/jats:italic>\n            at the right end of Tn4 was a truncated Tn\n            <jats:italic>1721<\/jats:italic>\n            . Restriction enzyme analysis showed that R plasmid pAr-32, isolated from\n            <jats:italic>A. salmonicida<\/jats:italic>\n            in Japan in 1970, had the same backbone structure as pRAS1, while the drug resistance-determining region contained a complex class 1 integron with an\n            <jats:italic>aadA2<\/jats:italic>\n            cassette; the chloramphenicol resistance gene\n            <jats:italic>catA2<\/jats:italic>\n            , as in In6 of pSa; and a duplicate of the 3\u2032 conserved segment of the integron.\n          <\/jats:p>","DOI":"10.1128\/aac.47.4.1285-1290.2003","type":"journal-article","created":{"date-parts":[[2003,3,24]],"date-time":"2003-03-24T19:46:16Z","timestamp":1048535176000},"page":"1285-1290","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen\n            <i>Aeromonas salmonicida<\/i>"],"prefix":"10.1128","volume":"47","author":[{"given":"Henning","family":"S\u00f8rum","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine M.","family":"L'Abe\u0301e-Lund","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asta","family":"Solberg","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Wold","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4194-4201.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"649","DOI":"10.2331\/suisan.52.649","volume":"52","year":"1986","unstructured":"Akashi, A., and T. Aoki. 1986. Characterization of transferable R plasmids from Aeromonas hydrophila. Bull. Jpn. Soc. Sci. Fish.52:649-655.","journal-title":"Bull. Jpn. Soc. Sci. Fish."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90597-I"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1348-0421.1972.tb00653.x","volume":"16","year":"1972","unstructured":"Aoki, T., S. Egusa, C. Yada, and T. Watanabe. 1972. Studies of drug resistance and R factors in bacteria from pond-cultured salmonids. I. Amago (Oncorhynchus rhodurus macrostomus) and Yamame (Oncorhynchus masou ishikawae). Jpn. J. Microbiol.16:233-238.","journal-title":"Jpn. J. Microbiol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0147-619X(86)90059-4","volume":"16","year":"1986","unstructured":"Aoki, T., Y. Mitoma, and J. H. Crosa. 1986. 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