{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:34:45Z","timestamp":1789990485450,"version":"4.0.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            strain showing broad-spectrum resistance to \u03b2-lactams, including expanded-spectrum cephalosporins and carbapenems, was isolated from a patient with a urinary tract infection at the University Hospital of Varese in northern Italy. The isolate was found to produce metallo-\u03b2-lactamase activity and to harbor a 50-kb plasmid, named pVA758, carrying a new\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP<\/jats:sub>\n            determinant, named\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-12<\/jats:sub>\n            . Plasmid pVA758 was not self-transferable by conjugation to either\n            <jats:italic>Escherichia coli<\/jats:italic>\n            or\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            but could be introduced by electroporation and maintained in the latter host, where it conferred resistance or decreased susceptibility to various \u03b2-lactams. The IMP-12 enzyme is quite divergent from other IMP variants: its closest relatives are IMP-8 and IMP-2 (89 and 88% sequence identity, respectively), and IMP-1 is 85% identical to IMP-12. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-12<\/jats:sub>\n            determinant is carried on an integron-borne gene cassette whose\n            <jats:italic>attC<\/jats:italic>\n            recombination site is related to those present in cassettes containing\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-1<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-6<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-7<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-10<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-11<\/jats:sub>\n            and unrelated to that present in cassettes containing\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-2<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-8<\/jats:sub>\n            . IMP-12 was overproduced in\n            <jats:italic>E. coli<\/jats:italic>\n            by using a T7-based expression system and was purified by cation-exchange chromatography followed by gel filtration. Kinetic analysis revealed that, like other IMP variants, IMP-12 exhibits an overall preference for cephalosporins and carbapenems rather than for penicillins and does not hydrolyze temocillin and aztreonam. However, IMP-12 also exhibits some notable functional differences from other IMP variants, including uniformly poor activity toward penicillins (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            \/\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            values, around 10\n            <jats:sup>4<\/jats:sup>\n            M\n            <jats:sup>\u22121<\/jats:sup>\n            \u00b7 s\n            <jats:sup>\u22121<\/jats:sup>\n            ) and a remarkably high\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            (around 900 \u03bcM) for imipenem.\n          <\/jats:p>","DOI":"10.1128\/aac.47.5.1522-1528.2003","type":"journal-article","created":{"date-parts":[[2003,4,22]],"date-time":"2003-04-22T21:03:23Z","timestamp":1051045403000},"page":"1522-1528","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["IMP-12, a New Plasmid-Encoded Metallo-\u03b2-Lactamase from a\n            <i>Pseudomonas putida<\/i>\n            Clinical Isolate"],"prefix":"10.1128","volume":"47","author":[{"given":"Jean-Denis","family":"Docquier","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Letizia","family":"Riccio","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mugnaioli","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Ospedale di Circolo and Universita\u0300 dell'Insubria, I-21100 Varese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Endimiani","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Ospedale di Circolo and Universita\u0300 dell'Insubria, I-21100 Varese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Toniolo","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Ospedale di Circolo and Universita\u0300 dell'Insubria, I-21100 Varese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100 L'Aquila, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.7.1612"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.710-714.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi992569m"},{"key":"e_1_3_2_5_2","first-page":"33","volume":"215","year":"2002","unstructured":"Da Silva, G. 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