{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:47:06Z","timestamp":1790236026146,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Clinical\n            <jats:italic>Oligella urethralis<\/jats:italic>\n            isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and expression experiments gave recombinant clones expressing either an AmpC \u03b2-lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing \u03b2-lactamase-type phenotype of resistance. The AmpC \u03b2-lactamase identified (ABA-1), which had a pI value of 8.2, had 98% amino acid identity with a chromosomally encoded cephalosporinase of\n            <jats:italic>Acinetobacter baumannii<\/jats:italic>\n            . A 820-bp insertion sequence element, IS\n            <jats:italic>Our1<\/jats:italic>\n            , belonging to the IS\n            <jats:italic>6<\/jats:italic>\n            family of insertion sequence elements, was identified immediately upstream of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ABA-1<\/jats:sub>\n            , providing a \u221235 promoter sequence and likely giving rise to a hybrid promoter region. The carbenicillin-hydrolyzing \u03b2-lactamase identified (CARB-8), which had a pI value of 6.4, differed from CARB-5 by two amino acid substitutions. Hybridization of\n            <jats:italic>Ceu<\/jats:italic>\n            I fragment I-restricted DNA fragments of\n            <jats:italic>O. urethralis<\/jats:italic>\n            COH-1 with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ABA-1<\/jats:sub>\n            -,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CARB-8<\/jats:sub>\n            -, and 16S rRNA-specific probes indicated the chromosomal integration of the \u03b2-lactamase genes. PCR and hybridization experiments failed to detect\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CARB-8<\/jats:sub>\n            - and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ABA-1<\/jats:sub>\n            -like genes in three\n            <jats:italic>O. urethralis<\/jats:italic>\n            reference strains, indicating that the \u03b2-lactamase genes identified were the source of acquired resistance in\n            <jats:italic>O. urethralis<\/jats:italic>\n            COH-1. This is one of the few examples of the interspecies transfer and the chromosomal integration of a gene encoding a naturally occurring \u03b2-lactamase.\n          <\/jats:p>","DOI":"10.1128\/aac.47.5.1536-1542.2003","type":"journal-article","created":{"date-parts":[[2003,4,22]],"date-time":"2003-04-22T21:03:23Z","timestamp":1051045403000},"page":"1536-1542","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Chromosomal Integration of a Cephalosporinase Gene from\n            <i>Acinetobacter baumannii<\/i>\n            into\n            <i>Oligella urethralis<\/i>\n            as a Source of Acquired Resistance to \u03b2-Lactams"],"prefix":"10.1128","volume":"47","author":[{"given":"Hedi","family":"Mammeri","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"},{"name":"Laboratoire Central, Ho\u0302pital Albert Chenevier, 94000 Cre\u0301teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Mangeney","sequence":"additional","affiliation":[{"name":"Laboratoire Central, Ho\u0302pital Albert Chenevier, 94000 Cre\u0301teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1924-1930.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/319858"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.428-432.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-36-2-228"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1110"},{"key":"e_1_3_2_9_2","first-page":"507","volume":"65","year":"1978","unstructured":"Buxton, A. 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