{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:22:04Z","timestamp":1793157724918,"version":"4.5.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            , the causative agent of melioidosis, is inherently resistant to a variety of antibiotics including aminoglycosides, macrolides, polymyxins, and \u03b2-lactam antibiotics. Despite resistance to many \u03b2-lactams, ceftazidime and \u03b2-lactamase inhibitor-\u03b2-lactam combinations are commonly used for treatment of melioidosis. Here, we examine the enzyme kinetics of \u03b2-lactamase isolated from mutants resistant to ceftazidime and clavulanic acid inhibition and describe specific mutations within conserved motifs of the \u03b2-lactamase enzyme which account for these resistance patterns. Sequence analysis of regions flanking the\n            <jats:italic>B. pseudomallei penA<\/jats:italic>\n            gene revealed a putative regulator gene located downstream of\n            <jats:italic>penA<\/jats:italic>\n            . We have cloned and sequenced the\n            <jats:italic>penA<\/jats:italic>\n            gene from\n            <jats:italic>B. mallei<\/jats:italic>\n            and found it to be identical to\n            <jats:italic>penA<\/jats:italic>\n            from\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.47.7.2082-2087.2003","type":"journal-article","created":{"date-parts":[[2003,6,23]],"date-time":"2003-06-23T19:17:06Z","timestamp":1056395826000},"page":"2082-2087","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":98,"title":["<i>Burkholderia pseudomallei<\/i>\n            Class A \u03b2-Lactamase Mutations That Confer Selective Resistance against Ceftazidime or Clavulanic Acid Inhibition"],"prefix":"10.1128","volume":"47","author":[{"given":"Chanwit","family":"Tribuddharat","sequence":"first","affiliation":[{"name":"Department of Microbiology, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N12"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N12"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald E.","family":"Woods","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N12"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-1-317"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/520218"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1132-1135.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(00)90333-X"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.1.52"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/28.2.321"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.874-877.1998"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.7.1608"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.8.1635"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2294"},{"key":"e_1_3_2_13_2","first-page":"410","volume":"29","year":"1998","unstructured":"Leelarasamee, A. 1998. Burkholderia pseudomallei: the unbeatable foe? Southeast Asian J. Trop. Med. Public Health29:410-415.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.313"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf165"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3355-3361.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.34-44.2001"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.4.1074"},{"key":"e_1_3_2_19_2","unstructured":"Schweitzer H. P. T. Klassen and T. Hoang. 1996. Improved methods for gene analysis and expression ion pseudomonas spp. p. 229-237. In T. Nakazawa K. Furukawa D. Haas and S. Silver (ed.) Molecular biology of pseudomonads. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/19.5.846"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.7.2082-2087.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.7.2082-2087.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T18:25:37Z","timestamp":1645467937000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.47.7.2082-2087.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1128\/AAC.47.7.2082-2087.2003"],"URL":"https:\/\/doi.org\/10.1128\/aac.47.7.2082-2087.2003","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]},"assertion":[{"value":"2002-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-03-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}