{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:38:58Z","timestamp":1773283138574,"version":"3.50.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Clinical isolates of\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            with reduced susceptibilities to cephalosporins were collected from 1993 to 2000. The organisms were screened for the extended-spectrum \u03b2-lactamase (ESBL) phenotype, and plasmid extracts were screened for genetic markers by hybridization. A\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            probe was derived from pUC19; other probes were derived from pACM1, the plasmid responsible for the first known appearance of an ESBL in our institution. These probes included\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            ,\n            <jats:italic>int<\/jats:italic>\n            ,\n            <jats:italic>aac(3)-Ia<\/jats:italic>\n            ,\n            <jats:italic>dfrA1<\/jats:italic>\n            , IS\n            <jats:italic>6100<\/jats:italic>\n            ,\n            <jats:italic>tetA<\/jats:italic>\n            , IncM markers, and Anon 13, a marker for the\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            chromosomal sequences that flank\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-5<\/jats:sub>\n            . There were 42 hybridization patterns among 237 isolates. Patterns designated pACM1-like occurred in 44% of the isolates (eight species) and were always associated with the clavulanic acid (CA)-susceptible ESBL phenotype. The TEM marker was not predictive of the ESBL phenotype. Mapping indicated the presence of an SHV marker and up to 7.5 kb of its flanking chromosomal sequences in three non-IncM plasmids obtained in transformation experiments. We theorize that this DNA segment spread to other plasmids from pACM1-like sources. CA insensitivity became more frequent with time and was usually associated with either the TEM marker or the absence of both\n            <jats:italic>bla<\/jats:italic>\n            markers. One plasmid-encoded enzyme with characteristics of an AmpC \u03b2-lactamase was observed in a transformant lacking both TEM and SHV markers. Although SHV type ESBLs were a continuing source of reduced susceptibility to cephalosporins in our institution, organisms with different resistance mechanisms were added to the hospital microflora in later years. These changes might be related, in part, to ESBL control strategies implemented in 1995.\n          <\/jats:p>","DOI":"10.1128\/aac.47.7.2179-2185.2003","type":"journal-article","created":{"date-parts":[[2003,6,23]],"date-time":"2003-06-23T19:17:06Z","timestamp":1056395826000},"page":"2179-2185","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Survey of Plasmid-Associated Genetic Markers in\n            <i>Enterobacteriaceae<\/i>\n            with Reduced Susceptibilities to Cephalosporins"],"prefix":"10.1128","volume":"47","author":[{"given":"Karen E.","family":"Preston","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen M.","family":"Graffunder","sequence":"additional","affiliation":[{"name":"Epidemiology, Albany Medical Center Hospital, Albany, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann M.","family":"Evans","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Venezia","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.11.1660"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.134.3.1141-1156.1978"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.2.500-510.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/BF02390679","volume":"13","year":"1994","unstructured":"Jacoby, G. A. 1994. Genetics of extended-spectrum \u03b2-lactamases. Eur. J. Clin. Microbiol. Infect. Dis.13(Suppl. 1):2-11.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1281"},{"key":"e_1_3_2_9_2","unstructured":"National Committee for Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests 6th ed. Approved standard M2-A6. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_10_2","unstructured":"National Committee for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial susceptibility testing; 12th informational supplement. Document M100-S12. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.1.1-11.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1997.1284"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1418"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.2000.1472"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1006\/plas.1999.1417","volume":"42","year":"1999","unstructured":"Preston, K. E., and R. A. Venezia. 1999. Restriction map and properties of Klebsiella oxytoca plasmid pACM1. Plasmid42:92-103.","journal-title":"Plasmid"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-619X(02)00017-3"},{"key":"e_1_3_2_17_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.4.915"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.10.2911-2914.2000"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.7.2179-2185.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.47.7.2179-2185.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T18:27:53Z","timestamp":1645468073000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.47.7.2179-2185.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1128\/AAC.47.7.2179-2185.2003"],"URL":"https:\/\/doi.org\/10.1128\/aac.47.7.2179-2185.2003","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]},"assertion":[{"value":"2002-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}