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When tested at extracellular concentrations equivalent to their maximum concentrations in human serum, all antibiotics except azithromycin caused a significant reduction of the postphagocytosis inoculum within 24 h, albeit to markedly different extents (telithromycin [2 mg\/liter], 0.60 log; ciprofloxacin [4.3 mg\/liter], 0.81 log; gentamicin [18 mg\/liter], 1.21 log; moxifloxacin [4 mg\/liter], 1.51 log; oritavancin [25 mg\/liter], 3.49 log). Intracellular activities were not systematically related to drug accumulation (apparent cellular-to-extracellular concentration ratios in infected cells: ciprofloxacin, 3.2; gentamicin, 6.8; telithromycin, 8.7; moxifloxacin, 13.4; azithromycin, 50; oritavancin, 348). Intracellular activity was not directly correlated to extracellular activity as measured in broth. Conditions of pH 5 (i.e., mimicking that of phagolysosomes) markedly reduced the activity of gentamicin, azithromycin, and telithromycin (\u226532\u00d7) and fairly extensively reduced that of ciprofloxacin and moxifloxacin (\u22654\u00d7) but did not affect oritavancin activity. We conclude that the cellular accumulation of antibiotics is not the only parameter to take into account for intracellular activity but that local environmental conditions (such as pH) and other factors can also prove critical.\n          <\/jats:p>","DOI":"10.1128\/aac.47.7.2283-2292.2003","type":"journal-article","created":{"date-parts":[[2003,6,23]],"date-time":"2003-06-23T19:17:06Z","timestamp":1056395826000},"page":"2283-2292","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":130,"title":["Quantitative Analysis of Gentamicin, Azithromycin, Telithromycin, Ciprofloxacin, Moxifloxacin, and Oritavancin (LY333328) Activities against Intracellular\n            <i>Staphylococcus aureus<\/i>\n            in Mouse J774 Macrophages"],"prefix":"10.1128","volume":"47","author":[{"given":"Cristina","family":"Seral","sequence":"first","affiliation":[{"name":"Unite\u0301 de Pharmacologie Cellulaire et Mole\u0301culaire, Universite\u0301 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Van Bambeke","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Pharmacologie Cellulaire et Mole\u0301culaire, Universite\u0301 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Tulkens","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Pharmacologie Cellulaire et Mole\u0301culaire, Universite\u0301 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100703"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02771843"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/41.6.655"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1159\/000238988","volume":"39","year":"1993","unstructured":"Anderson, R., and G. 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