{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:48:35Z","timestamp":1773658115252,"version":"3.50.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Resistance to extended-spectrum cephalosporins is often associated with plasmid encoded extended spectrum \u03b2-lactamases (ESBL). In order to evaluate the prevalence and diversity of ESBLs in enterobacteria in our city, a 1-month-period survey was carried out from April to May 2000. Extended-spectrum-cephalosporin-resistant strains, isolated from inpatient clinical specimens other than stools, were collected among 17 participating hospitals. From a total of 427 enterobacterial strains that were collected during this period, 39 were extended-spectrum cephalosporin resistant. The National Committee for Clinical Laboratory Standards' Screening and Confirmatory Tests for ESBL production were performed using cefotaxime and ceftazidime; cefepime and cefepime-clavulanic acid-containing disks were included. \u03b2-Lactamases were characterized by isoelectric focusing and PCR amplification using specific primers. Three different ESBLs were detected: SHV-related (4 isolates), PER-2-type (9 isolates), and CTX-M-2-related (26 isolates). Sequencing of the corresponding genes confirmed CTX-M-2 in 19 of 21 and CTX-M-31 (an allelic variant) in the remaining 2 of 21. CTX-M-2 (or its variant) was detected in all\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Enterobacter aerogenes<\/jats:italic>\n            ,\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            ,\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            , and\n            <jats:italic>Providencia stuartii<\/jats:italic>\n            strains, while PER-2 was detected in\n            <jats:italic>Enterobacter cloacae<\/jats:italic>\n            ,\n            <jats:italic>E. aerogenes<\/jats:italic>\n            , and\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            ; SHV-related ESBL were found only in\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            . These results clearly show that CTX-M-2 is the most prevalent ESBL produced by enterobacterial species isolated from public hospitals in Buenos Aires.\n          <\/jats:p>","DOI":"10.1128\/aac.47.9.2864-2867.2003","type":"journal-article","created":{"date-parts":[[2003,8,22]],"date-time":"2003-08-22T21:07:12Z","timestamp":1061586432000},"page":"2864-2867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Extended-Spectrum \u03b2-Lactamases in\n            <i>Enterobacteriaceae<\/i>\n            in Buenos Aires, Argentina, Public Hospitals"],"prefix":"10.1128","volume":"47","author":[{"given":"M.","family":"Quinteros","sequence":"first","affiliation":[{"name":"Hospital de Enfermedades Infecciosas \u201cF. J. 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Mun\u0303iz\u201d"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Korbenfeld","sequence":"additional","affiliation":[{"name":"Hospital de Enfermedades Infecciosas \u201cF. J. Mun\u0303iz\u201d"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Couto","sequence":"additional","affiliation":[{"name":"Hospital de Enfermedades Infecciosas \u201cF. J. 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