{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T07:07:49Z","timestamp":1793516869583,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Klebsiella pneumoniae<\/jats:italic>strain 11978 was isolated in Turkey in 2001 and was found to be resistant to all \u03b2-lactams, including carbapenems. Cloning and expression in<jats:italic>Escherichia coli<\/jats:italic>identified five \u03b2-lactamases, including two novel oxacillinases. The \u03b2-lactamase OXA-48 hydrolyzed imipenem at a high level and was remotely related (less than 46% amino acid identity) to the other oxacillinases. It hydrolyzed penicillins and imipenem but not expanded-spectrum cephalosporins. The<jats:italic>bla<\/jats:italic><jats:sub>OXA-48<\/jats:sub>gene was plasmid encoded and not associated with an integron, in contrast to most of the oxacillinase genes. An insertion sequence, IS<jats:italic>1999<\/jats:italic>, was found immediately upstream of<jats:italic>bla<\/jats:italic><jats:sub>OXA-48<\/jats:sub>. Another plasmid that encoded a second oxacillinase gene,<jats:italic>bla<\/jats:italic><jats:sub>OXA-47<\/jats:sub>, located inside a class 1 integron was identified in<jats:italic>K. pneumoniae<\/jats:italic>11978. OXA-47 had a narrow spectrum of hydrolysis activity and did not hydrolyze ceftazidime or imipenem, as is found for the \u03b2-lactamase (OXA-1) to which it is related. In addition, \u03b2-lactamases TEM-1 and SHV-2a were expressed from the same<jats:italic>K. pneumoniae<\/jats:italic>isolate. Analysis of the outer membrane proteins of this isolate revealed that it lacked a porin of ca. 36 kDa. Thus, the high-level resistance to \u03b2-lactams of this clinical isolate resulted from peculiar \u03b2-lactamases and modification of outer membrane proteins.<\/jats:p>","DOI":"10.1128\/aac.48.1.15-22.2004","type":"journal-article","created":{"date-parts":[[2003,12,24]],"date-time":"2003-12-24T02:03:22Z","timestamp":1072231402000},"page":"15-22","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":912,"title":["Emergence of Oxacillinase-Mediated Resistance to Imipenem in<i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Laurent","family":"Poirel","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Universite\u0301 Paris XI, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"He\u0301ritier","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Universite\u0301 Paris XI, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venus","family":"Tolu\u0308n","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Istanbul Medical Faculty, Capa, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Universite\u0301 Paris XI, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.583-588.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.7.1636"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.6.1615-1620.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2510073"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.2004-2006.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1556-1561.2000"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.563"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb00894.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.11.3679-3682.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.196-199.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/338786"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.268-273.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-145-3-673"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(84)90063-5"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)75604-X"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2920555"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6874"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)62567-6"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1669"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.Supplement_1.S19"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"865","DOI":"10.2174\/1381612805666230112185101","volume":"5","year":"1999","unstructured":"Naas, T., and P. Nordmann. 1999. OXA-type \u03b2-lactamases. Curr. Pharm. Design5:865-879.","journal-title":"Curr. Pharm. Design"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1281"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13691.x"},{"key":"e_1_3_2_25_2","unstructured":"National Committee for Clinical Laboratory Standards. 2001. Performance standards for antimicrobial susceptibility testing. Eleventh informational supplement. M100-S11. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7378"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0924-8579(93)90045-7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0732-8893(99)00054-1","volume":"35","year":"1999","unstructured":"Pfaller, M. A., V. Korten, R. N. Jones, G. V. Doern, et al. 1999. Multicenter evaluation of the antimicrobial activity for seven broad-spectrum \u03b2-lactams in Turkey using the Etest method. Diagn. Microbiol. Infect. Dis.35:65-73.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.1.1-11.2002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2188"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.10.2557"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.447-453.2001"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.755-758.2003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.3.573"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389201023378427"},{"key":"e_1_3_2_36_2","unstructured":"Sambrook J. and D. W. Russell. 2001. Molecular cloning: a laboratory manual 3rd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.11.3422-3427.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1993.1004"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2368-2371.2001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.1.15-22.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.1.15-22.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T17:29:10Z","timestamp":1682616550000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.48.1.15-22.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/AAC.48.1.15-22.2004"],"URL":"https:\/\/doi.org\/10.1128\/aac.48.1.15-22.2004","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}