{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:39:00Z","timestamp":1791182340961,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A vancomycin-resistant\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and analysis of 16S ribosomal DNA,\n            <jats:italic>gyrA<\/jats:italic>\n            , and\n            <jats:italic>gyrB<\/jats:italic>\n            sequences; all of the results were consistent with the\n            <jats:italic>S. aureus<\/jats:italic>\n            identification. The MICs of a variety of antimicrobial agents were determined by broth microdilution and macrodilution methods following National Committee for Clinical Laboratory Standards (NCCLS) guidelines. The isolate was resistant to vancomycin (MIC = 32 \u03bcg\/ml), aminoglycosides, \u03b2-lactams, fluoroquinolones, macrolides, and tetracycline, but it was susceptible to linezolid, minocycline, quinupristin-dalfopristin, rifampin, teicoplanin, and trimethoprim-sulfamethoxazole. The isolate, which was originally detected by using disk diffusion and a vancomycin agar screen plate, was vancomycin susceptible by automated susceptibility testing methods. Pulsed-field gel electrophoresis (PFGE) of\n            <jats:italic>Sma<\/jats:italic>\n            I-digested genomic DNA indicated that the isolate belonged to the USA100 lineage (also known as the New York\/Japan clone), the most common staphylococcal PFGE type found in hospitals in the United States. The VRSA isolate contained two plasmids of 120 and 4 kb and was positive for\n            <jats:italic>mecA<\/jats:italic>\n            and\n            <jats:italic>vanA<\/jats:italic>\n            by PCR amplification. The\n            <jats:italic>vanA<\/jats:italic>\n            sequence was identical to the\n            <jats:italic>vanA<\/jats:italic>\n            sequence present in Tn\n            <jats:italic>1546<\/jats:italic>\n            . A DNA probe for\n            <jats:italic>vanA<\/jats:italic>\n            hybridized to the 120-kb plasmid. This is the second VRSA isolate reported in the United States.\n          <\/jats:p>","DOI":"10.1128\/aac.48.1.275-280.2004","type":"journal-article","created":{"date-parts":[[2003,12,24]],"date-time":"2003-12-24T02:03:22Z","timestamp":1072231402000},"page":"275-280","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":283,"title":["Vancomycin-Resistant\n            <i>Staphylococcus aureus<\/i>\n            Isolate from a Patient in Pennsylvania"],"prefix":"10.1128","volume":"48","author":[{"given":"Fred C.","family":"Tenover","sequence":"first","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Weigel","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Appelbaum","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda K.","family":"McDougal","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Chaitram","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrid","family":"McAllister","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancye","family":"Clark","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Killgore","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.","family":"O'Hara","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Jevitt","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean B.","family":"Patel","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu\u0308lent","family":"Bozdogan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.1.117-127.1993"},{"key":"e_1_3_2_3_2","unstructured":"Bannerman T. 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