{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:19:38Z","timestamp":1791688778052,"version":"4.3.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enterobacterial strains of\n            <jats:italic>Raoultella<\/jats:italic>\n            spp. display a penicillinase-related \u03b2-lactam resistance pattern suggesting the presence of a chromosomal\n            <jats:italic>bla<\/jats:italic>\n            gene. From whole-cell DNA of\n            <jats:italic>Raoultella planticola<\/jats:italic>\n            strain ATCC 33531\n            <jats:sup>T<\/jats:sup>\n            and\n            <jats:italic>Raoultella ornithinolytica<\/jats:italic>\n            strain ATCC 31898\n            <jats:sup>T<\/jats:sup>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            genes were cloned and expressed into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Each gene encoded an Ambler class A \u03b2-lactamase, named PLA-1 and ORN-1 for\n            <jats:italic>R. planticola<\/jats:italic>\n            and\n            <jats:italic>R. ornithinolytica<\/jats:italic>\n            , respectively. These \u03b2-lactamases (291 amino acids), with the same pI value of 7.8, had a shared amino acid identity of 94%, 37 to 47% identity with the majority of the chromosome-encoded class A \u03b2-lactamases previously described for\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            , and 66 to 69% identity with the two \u03b2-lactamases LEN-1 and SHV-1 from\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            . However, the highest identity percentage (69 to 71%) was found with the plasmid-mediated \u03b2-lactamase TEM-1. PLA-1, which displayed very strong hydrolytic activity against penicillins, also displayed significant hydrolytic activity against cefepime and, to a lesser extent, against cefotaxime and aztreonam, but there was no hydrolytic activity against ceftazidime. Such a substrate profile suggests that the\n            <jats:italic>Raoultella<\/jats:italic>\n            \u03b2-lactamases PLA-1 and ORN-1 should be classified into the group 2be of the \u03b2-lactamase classification of K. Bush, G. A. Jacoby, and A. A. Medeiros (Antimicrob. Agents Chemother.\n            <jats:bold>39:<\/jats:bold>\n            1211-1233, 1995). The highly homologous regions upstream of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>PLA-1A<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ORN-1A<\/jats:sub>\n            genes comprised a nucleotide sequence identical to the \u221235 region and another one very close to the \u221210 region of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>LEN-1<\/jats:sub>\n            gene. From now on, as the\n            <jats:italic>bla<\/jats:italic>\n            gene sequences of the most frequent\n            <jats:italic>Raoultella<\/jats:italic>\n            and\n            <jats:italic>Klebsiella<\/jats:italic>\n            species are available, the\n            <jats:italic>bla<\/jats:italic>\n            gene amplification method can be used to differentiate these species from each other, which the biochemical tests currently carried out in the clinical laboratory are unable to do.\n          <\/jats:p>","DOI":"10.1128\/aac.48.1.305-312.2004","type":"journal-article","created":{"date-parts":[[2003,12,24]],"date-time":"2003-12-24T02:03:22Z","timestamp":1072231402000},"page":"305-312","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Genetic and Biochemical Characterization of the Chromosomal Class A \u03b2-Lactamases of\n            <i>Raoultella<\/i>\n            (formerly\n            <i>Klebsiella<\/i>\n            )\n            <i>planticola<\/i>\n            and\n            <i>Raoultella ornithinolytica<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Estelle","family":"Walckenaer","sequence":"first","affiliation":[{"name":"Service de Microbiologie-Hygie\u0300ne, UFR Me\u0301dicale Paris-Ile-de-France Ouest, Universite\u0301 de Versailles-Saint Quentin-en-Yvelines, Versailles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, UFR Me\u0301dicale Paris-Sud, Ho\u0302pital de Bice\u0302tre (AP-HP), Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ve\u0301ronique","family":"Leflon-Guibout","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Ambroise Pare\u0301 (AP-HP), Boulogne-Billancourt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, UFR Me\u0301dicale Paris-Sud, Ho\u0302pital de Bice\u0302tre (AP-HP), Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-He\u0301le\u0300ne","family":"Nicolas-Chanoine","sequence":"additional","affiliation":[{"name":"Service de Microbiologie-Hygie\u0300ne, UFR Me\u0301dicale Paris-Ile-de-France Ouest, Universite\u0301 de Versailles-Saint Quentin-en-Yvelines, Versailles"},{"name":"Ho\u0302pital Ambroise Pare\u0301 (AP-HP), Boulogne-Billancourt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(03)00021-9"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569013"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2965-2968.2001"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3595-3598.2001"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-3-925"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2247-2253.2000"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1740-1742.2003"},{"key":"e_1_3_1_12_2","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/ijs.0.02408-0","volume":"53","year":"2003","unstructured":"Granier, S. 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