{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:54:18Z","timestamp":1791248058319,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2004,11,1]],"date-time":"2004-11-01T00:00:00Z","timestamp":1099267200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The sequence of the 45.2-kb multidrug and mercury resistance region of pRMH760, a large plasmid from a clinical isolate of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            collected in 1997 in Australia, was completed. Most of the modules found in the resistance determinant (r-det), or Tn\n            <jats:italic>2670<\/jats:italic>\n            , region of NR1 (also known as R100), isolated from a\n            <jats:italic>Shigella flexneri<\/jats:italic>\n            strain in Japan in the late 1950s, were present in pRMH760 but in a different configuration. The location was also different, with the Tn\n            <jats:italic>2670<\/jats:italic>\n            -derived region flanked by the transposition module of Tn\n            <jats:italic>1696<\/jats:italic>\n            and a mercury resistance module almost identical to one found in the plasmid pDU1358. This arrangement is consistent with a three-step process. First, the r-det was circularized via homologous recombination between the IS\n            <jats:italic>1<\/jats:italic>\n            elements and reincorporated at a new location, possibly in a different plasmid, via homologous recombination between the 5\u2032-conserved (5\u2032-CS) or 3\u2032-CS of the In34 integron in the r-det and the same region of a second class 1 integron in a Tn\n            <jats:italic>1696<\/jats:italic>\n            relative. Subsequently, resolvase-mediated recombination between the\n            <jats:italic>res<\/jats:italic>\n            sites in the r-det and a second mercury resistance transposon removed one end of the Tn\n            <jats:italic>1696<\/jats:italic>\n            -like transposon and part of the second transposon. Other events occurring within the r-det-derived portion have also contributed to the formation of the pRMH760 resistance region. Tn\n            <jats:italic>2<\/jats:italic>\n            or a close relative that includes the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1b<\/jats:sub>\n            gene had moved into the Tn\n            <jats:italic>21<\/jats:italic>\n            mercury resistance module with subsequent deletion of the adjacent sequence, and all four 38-bp inverted repeats corresponding to Tn\n            <jats:italic>21<\/jats:italic>\n            family transposon termini have been interrupted by an IS\n            <jats:italic>4321<\/jats:italic>\n            -like element.\n          <\/jats:p>","DOI":"10.1128\/aac.48.11.4250-4255.2004","type":"journal-article","created":{"date-parts":[[2004,10,25]],"date-time":"2004-10-25T18:09:00Z","timestamp":1098727740000},"page":"4250-4255","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Complex Multiple Antibiotic and Mercury Resistance Region Derived from the r-det of NR1 (R100)"],"prefix":"10.1128","volume":"48","author":[{"given":"Sally R.","family":"Partridge","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Macquarie University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth M.","family":"Hall","sequence":"additional","affiliation":[{"name":"School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/282864a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.15.4429-4437.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/BF00431594","volume":"153","year":"1977","unstructured":"Chandler, M., B. Allet, E. Gallay, E. Boy de La Tour, and L. Caro. 1977. Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1. Mol. Gen. Genet.153:289-295.","journal-title":"1. Mol. Gen. Genet."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1101\/SQB.1979.043.01.139","volume":"43","year":"1979","unstructured":"Chandler, M., L. Silver, D. Lane, and L. Caro. 1979. Properties of an autonomous r-determinant from R100.1. Cold Spring Harb. Symp. Quant. Biol.43:1223-1231.","journal-title":"Symp. Quant. Biol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.2.913-916.1987"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90540-R"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.10.3112"},{"key":"e_1_3_2_9_2","first-page":"272","year":"2002","unstructured":"Grindley, N. D. F. 2002. 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Academic Press, London, United Kingdom.","journal-title":"Encyclopedia of genetics"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-7646(98)80026-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.24.5712"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.150.3.1266-1273.1982"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90228-9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.11.2991-3000.2001"},{"key":"e_1_3_2_17_2","first-page":"413","volume":"126","year":"1981","unstructured":"Iida, S., C. H\u00e4nni, C. Echarti, and W. Arber. 1981. Is the IS1-flanked r-determinant of the R plasmid NR1 a transposon? J. Gen. Microbiol.126:413-425.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.3201\/eid0912.030172","volume":"9","year":"2003","unstructured":"Izumiya, H., N. Nojiri, Y. 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