{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:42:39Z","timestamp":1789522959119,"version":"build-2803163510"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A combination of biochemical and genetic experiments were performed in order to better understand the mechanism of expression of high-level vancomycin resistance in\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    . The transcription of\n                    <jats:italic>pbp2<\/jats:italic>\n                    of the highly vancomycin- and oxacillin-resistant strain COLVA\n                    <jats:sub>200<\/jats:sub>\n                    and its mutant derivative with inactivated\n                    <jats:italic>mecA<\/jats:italic>\n                    were put under the control of an inducible promoter, and the dependence of oxacillin and vancomycin resistance and cell wall composition on the concentration of the isopropyl-\u03b2-\n                    <jats:sc>d<\/jats:sc>\n                    -thiogalactopyranoside inducer was determined. The results indicate that\n                    <jats:italic>mecA\u2014<\/jats:italic>\n                    the genetic determinant of oxacillin resistance\u2014while essential for oxacillin resistance, is not involved with the expression of vancomycin resistance. Penicillin binding protein 2A, the protein product of\n                    <jats:italic>mecA<\/jats:italic>\n                    , appears to be unable to utilize the depsipeptide cell wall precursor produced in the vancomycin-resistant cells for transpeptidation. The key penicillin binding protein essential for vancomycin resistance and for the synthesis of the abnormally structured cell walls characteristic of vancomycin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (A. Severin, K. Tabei, F. Tenover, M. Chung, N. Clarke, and A. Tomasz, J. Biol. Chem.\n                    <jats:italic>279<\/jats:italic>\n                    :3398-3407, 2004) is penicillin binding protein 2.\n                  <\/jats:p>","DOI":"10.1128\/aac.48.12.4566-4573.2004","type":"journal-article","created":{"date-parts":[[2004,11,23]],"date-time":"2004-11-23T22:00:46Z","timestamp":1101247246000},"page":"4566-4573","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Penicillin-Binding Protein 2 Is Essential for Expression of High-Level Vancomycin Resistance and Cell Wall Synthesis in Vancomycin- Resistant\n                    <i>Staphylococcus aureus<\/i>\n                    Carrying the Enterococcal\n                    <i>vanA<\/i>\n                    Gene Complex"],"prefix":"10.1128","volume":"48","author":[{"given":"Anatoly","family":"Severin","sequence":"first","affiliation":[{"name":"The Rockefeller University, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Tabei","sequence":"additional","affiliation":[{"name":"Wyeth, Pearl River, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Tomasz","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01114.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.375-381.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.15.4696-4703.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.14.9910"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00107a007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa025025"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49903-1"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90451-6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.158.2.513-516.1984"},{"key":"e_1_3_2_11_2","first-page":"870","volume":"9","year":"2002","unstructured":"Lim, D., and N. C. J. Strynadka. 2002. Structural basis for the beta-lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Nat. Struct. Biol.9:870-876.","journal-title":"Nat. Struct. Biol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.9.1777"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74310-5"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6525-6531.2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.23.6077-6081.1998"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.191260798"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1997.3.409"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309593200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.8.2557-2566.1997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.12.3223"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1974.tb43278.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.1.124"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090956"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.12.4566-4573.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.12.4566-4573.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T13:17:04Z","timestamp":1645449424000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.48.12.4566-4573.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1128\/AAC.48.12.4566-4573.2004"],"URL":"https:\/\/doi.org\/10.1128\/aac.48.12.4566-4573.2004","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12]]},"assertion":[{"value":"2004-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}