{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:30:57Z","timestamp":1776918657154,"version":"3.51.2"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Since January 2002\n            <jats:italic>Pseudomonas<\/jats:italic>\n            sp. strains resistant to carbapenems and ceftazidime have been routinely screened as part of the SENTRY Antimicrobial Surveillance Program for metallo-\u03b2-lactamase production, and their resistance determinants have been analyzed.\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            index strain 101-4704, which harbors a novel\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP<\/jats:sub>\n            variant,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-16<\/jats:sub>\n            , was isolated in April 2002 from a 60-year-old man in Brasi\u0301lia, Brazil.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-16<\/jats:sub>\n            was found on the chromosome of the\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            index strain, and the deduced amino acid sequence (IMP-16) showed the greatest identities to IMP-11 (90.3%) and IMP-8 (89.5%). Sequence analysis revealed that\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-16<\/jats:sub>\n            was associated with a class 1 integron, which also encoded aminoglycoside-modifying enzymes. Downstream of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-16<\/jats:sub>\n            resided an open reading frame, which consisted of a new aminoglycoside-modifying gene, namely,\n            <jats:italic>aac(6\u2032)-30<\/jats:italic>\n            , which was fused with\n            <jats:italic>aac(6\u2032)-Ib\u2032<\/jats:italic>\n            . The amino acid sequence of the\n            <jats:italic>aac(6\u2032)-30<\/jats:italic>\n            putative protein showed the most identity (52.7%) to the sequence of AAC(6\u2032)-29b described previously. The fourth gene cassette constituted\n            <jats:italic>aadA1<\/jats:italic>\n            . The steady-state kinetics of IMP-16 demonstrated that the enzyme preferred cephalosporins and carbapenems to penicillins. The main functional difference observed among the kinetic values for IMP-16 compared to those for other IMPs was a lack of cefoxitin hydrolysis and a lower\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            \/\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            value for imipenem (0.36 \u03bcM\n            <jats:sup>\u22121<\/jats:sup>\n            \u2009\u00b7\u2009s\n            <jats:sup>\u22121<\/jats:sup>\n            ). This report further emphasizes the spread of metallo-\u03b2-lactamase genes and their close association with various aminoglycoside resistance genes.\n          <\/jats:p>","DOI":"10.1128\/aac.48.12.4693-4702.2004","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T03:00:46Z","timestamp":1101265246000},"page":"4693-4702","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Integron Carrying a Novel Metallo-\u03b2-Lactamase Gene,\n            <i>bla<\/i>\n            <sub>IMP-16<\/sub>\n            , and a Fused Form of Aminoglycoside-Resistant Gene\n            <i>aac(6\u2032)-30\/aac(6\u2032)-Ib\u2032<\/i>\n            : Report from the SENTRY Antimicrobial Surveillance Program"],"prefix":"10.1128","volume":"48","author":[{"given":"Rodrigo E.","family":"Mendes","sequence":"first","affiliation":[{"name":"Disciplina de Doenc\u0327as Infecciosas e Parasita\u0301rias, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo"},{"name":"Department of Pathology &amp; Microbiology, University of Bristol, Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Toleman","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Microbiology, University of Bristol, Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julival","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Hospital de Base do Distrito Federal, Brasi\u0301lia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helio S.","family":"Sader","sequence":"additional","affiliation":[{"name":"Disciplina de Doenc\u0327as Infecciosas e Parasita\u0301rias, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo"},{"name":"The JONES Group\/JMI Laboratories, North Liberty, Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald N.","family":"Jones","sequence":"additional","affiliation":[{"name":"The JONES Group\/JMI Laboratories, North Liberty, Iowa"},{"name":"Tufts University School of Medicine, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy R.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Microbiology, University of Bristol, Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.583-588.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg270"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/514922"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.710-714.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.3017-3026.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)05954-6"},{"key":"e_1_3_2_8_2","first-page":"33","volume":"215","year":"2002","unstructured":"Da Silva, G. 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