{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:13:12Z","timestamp":1791421992763,"version":"4.3.3"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    , the causative agent of melioidosis, is intrinsically resistant to a wide range of antimicrobial agents, including \u03b2-lactams, aminoglycosides, macrolides, and polymyxins. An operon,\n                    <jats:italic>bpeR-bpeA-bpeB-oprB<\/jats:italic>\n                    , which encodes a putative repressor, a membrane fusion protein, an inner membrane protein, and an outer membrane protein, respectively, of a multidrug efflux pump of the resistance-nodulation-division family was identified in\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    . The divergently transcribed\n                    <jats:italic>bpeR<\/jats:italic>\n                    gene encodes a putative repressor protein of the TetR family which probably regulates the expression of the\n                    <jats:italic>bpeAB<\/jats:italic>\n                    -\n                    <jats:italic>oprB<\/jats:italic>\n                    gene cluster. Comparison of the MICs and minimal bactericidal concentrations of antimicrobials for\n                    <jats:italic>bpeAB<\/jats:italic>\n                    deletion mutant KHW\u0394\n                    <jats:italic>bpeAB<\/jats:italic>\n                    and its isogenic wild-type parent, KHW, showed that the\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    BpeAB-OprB pump is responsible for the efflux of the aminoglycosides gentamicin and streptomycin, the macrolide erythromycin, and the dye acriflavine. Antibiotic efflux by the BpeAB-OprB pump was dependent on a proton gradient and differs from that by the AmrAB-OprA pump in that it did not efflux the aminoglycoside spectinomycin or the macrolide clarithromycin. The broad-spectrum efflux pump inhibitor MC-207,110 did not potentiate the effectiveness of the antimicrobials erythromycin and streptomycin in\n                    <jats:italic>B. pseudomallei.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1128\/aac.48.4.1128-1135.2004","type":"journal-article","created":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T15:18:01Z","timestamp":1080314281000},"page":"1128-1135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":98,"title":["BpeAB-OprB, a Multidrug Efflux Pump in\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Y. Y.","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore 117597, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. M. C.","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore 117597, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. M.","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore 117597, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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