{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:50:22Z","timestamp":1790707822935,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This report describes a study carried out to gain baseline information on the molecular characteristics of extended-spectrum beta-lactamase (ESBL)-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp. in Canada. A total of 29,323\n            <jats:italic>E. coli<\/jats:italic>\n            and 5,156\n            <jats:italic>Klebsiella<\/jats:italic>\n            sp. isolates were screened at 12 participating sites. Of these, 505 clinically significant, nonrepeat isolates displaying reduced susceptibility to the NCCLS-recommended beta-lactams were submitted to a central laboratory over a 1-year period ending on 30 September 2000. A total of 116 isolates were confirmed to be ESBL producers. PCR and sequence analysis revealed the presence of TEM-11 (\n            <jats:italic>n<\/jats:italic>\n            = 1), TEM-12 (\n            <jats:italic>n<\/jats:italic>\n            = 1), TEM-29 (\n            <jats:italic>n<\/jats:italic>\n            = 1), TEM-52 (\n            <jats:italic>n<\/jats:italic>\n            = 4), CTX-M-13 (\n            <jats:italic>n<\/jats:italic>\n            = 1), CTX-M-14 (\n            <jats:italic>n<\/jats:italic>\n            = 15), CTX-M-15 (\n            <jats:italic>n<\/jats:italic>\n            = 11), SHV-2 (\n            <jats:italic>n<\/jats:italic>\n            = 2), SHV-2a (\n            <jats:italic>n<\/jats:italic>\n            = 12), SHV-5 (\n            <jats:italic>n<\/jats:italic>\n            = 6), SHV-12 (\n            <jats:italic>n<\/jats:italic>\n            = 45), and SHV-30 (\n            <jats:italic>n<\/jats:italic>\n            = 2). Five novel beta-lactamases were identified and designated TEM-115 (\n            <jats:italic>n<\/jats:italic>\n            = 2), TEM-120 (\n            <jats:italic>n<\/jats:italic>\n            = 1), SHV-40 (\n            <jats:italic>n<\/jats:italic>\n            = 2), SHV-41 (\n            <jats:italic>n<\/jats:italic>\n            = 4), and SHV-42 (\n            <jats:italic>n<\/jats:italic>\n            = 1). In addition, no molecular mechanism was identified for five isolates displaying an ESBL phenotype. Macrorestriction analysis of all ESBL isolates was conducted, as was restriction fragment length polymorphism analysis of plasmids harboring ESBLs. Although a \u201cclonal\u201d distribution of isolates was observed at some individual sites, there was very little evidence suggesting intrahospital spread. In addition, examples of identical or closely related plasmids that were identified at geographically distinct sites across Canada are given. However, there was considerable diversity with respect to plasmid types observed.\n          <\/jats:p>","DOI":"10.1128\/aac.48.4.1204-1214.2004","type":"journal-article","created":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T20:18:01Z","timestamp":1080332281000},"page":"1204-1214","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":114,"title":["Ambler Class A Extended-Spectrum Beta-Lactamase-Producing\n            <i>Escherichia coli<\/i>\n            and\n            <i>Klebsiella<\/i>\n            spp. in Canadian Hospitals"],"prefix":"10.1128","volume":"48","author":[{"given":"Michael R.","family":"Mulvey","sequence":"first","affiliation":[{"name":"Nosocomial Infections, National Microbiology Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Bryce","sequence":"additional","affiliation":[{"name":"The Vancouver General Hospital, Vancouver, British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Boyd","sequence":"additional","affiliation":[{"name":"Nosocomial Infections, National Microbiology Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Ofner-Agostini","sequence":"additional","affiliation":[{"name":"The University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Christianson","sequence":"additional","affiliation":[{"name":"Nosocomial Infections, National Microbiology Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew E.","family":"Simor","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley","family":"Paton","sequence":"additional","affiliation":[{"name":"Division of Nosocomial and Occupational Infections, Centre for Infectious Disease Prevention and Control, Health Canada, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"203","volume":"134","year":"1995","unstructured":"Arlet, G., G. Brami, D. Decre, A. Flippo, O. Gaillot, P. H. Lagrange, and A. Philippon. 1995. Molecular characterisation by PCR-restriction fragment length polymorphism of TEM beta-lactamases. FEMS Microbiol. Lett.134:203-208.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4030-4036.2002"},{"key":"e_1_3_2_5_2","first-page":"185","volume":"174","year":"1999","unstructured":"Bou, G., J. Mart\u00ednez-Beltr\u00e1n, G. Cerver\u00f3, and J. C. P\u00e9rez-D\u00edaz. 1999. Biochemical and genetic characteristics of TEM-29B, a novel extended spectrum \u03b2-lactamase. FEMS Microbiol. Lett.174:185-190.","journal-title":"FEMS Microbiol. 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