{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:09:08Z","timestamp":1790968148914,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Among methicillin-resistant\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates, a staphylococcal chromosomal cassette containing the\n            <jats:italic>mecA<\/jats:italic>\n            gene (SCC\n            <jats:italic>mec<\/jats:italic>\n            ) is integrated into the chromosome at a unique site. SCC\n            <jats:italic>mec<\/jats:italic>\n            also contains unique\n            <jats:italic>ccrAB<\/jats:italic>\n            recombinase genes mediating its integration and excision from the genome and is flanked by characteristic left and right direct- and inverted-repeat sequences. A few non-\n            <jats:italic>mecA<\/jats:italic>\n            -containing SCC elements that have the other molecular features described above have recently been described. The origin of these cassettes is not clear. We have identified two new members of the SCC family integrated within\n            <jats:italic>orfX<\/jats:italic>\n            in\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            strain ATCC 12228, neither of which carries\n            <jats:italic>mecA<\/jats:italic>\n            . One is a 57-kb element flanked by a unique 28-bp SCC direct repeat. It was called the SCC composite island (SCC-CI) because it carries a 19-kb SCC element (SCC\n            <jats:italic>pbp4<\/jats:italic>\n            ) nested within it. SCC\n            <jats:italic>pbp4<\/jats:italic>\n            contains\n            <jats:italic>pbp4<\/jats:italic>\n            and\n            <jats:italic>tagF<\/jats:italic>\n            genes, as well as one pair of\n            <jats:italic>ccrAB<\/jats:italic>\n            genes (allotype 2) flanked by classical SCC-specific terminal repeats. External to SCC\n            <jats:italic>pbp4<\/jats:italic>\n            , SCC-CI contains a second pair of\n            <jats:italic>ccrAB<\/jats:italic>\n            genes (allotype 4), three IS\n            <jats:italic>431<\/jats:italic>\n            elements, and genes mediating resistance to heavy metals. Genes mediating restriction-modification that may facilitate horizontal transfer are also present within SCC-CI, both within and outside SCC\n            <jats:italic>pbp4.<\/jats:italic>\n            Several novel arrangements of the SCC direct and inverted repeats were identified. Several long stretches of homology with other SCCs were found within and outside SCC\n            <jats:italic>pbp4<\/jats:italic>\n            . In view of the fact that SCC-CI was found in a commensal species, it may represent a reservoir for sequences involved in genetic shuffling between staphylococci and may contribute to the diversity found in SCC elements.\n          <\/jats:p>","DOI":"10.1128\/aac.48.5.1823-1836.2004","type":"journal-article","created":{"date-parts":[[2004,4,22]],"date-time":"2004-04-22T19:58:16Z","timestamp":1082663896000},"page":"1823-1836","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["Novel Non-\n            <i>mecA<\/i>\n            -Containing Staphylococcal Chromosomal Cassette Composite Island Containing\n            <i>pbp4<\/i>\n            and\n            <i>tagF<\/i>\n            Genes in a Commensal Staphylococcal Species: a Possible Reservoir for Antibiotic Resistance Islands in\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Kanokporn","family":"Mongkolrattanothai","sequence":"first","affiliation":[{"name":"Section of Pediatric Infectious Diseases, Department of Pediatrics, University of Chicago, Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Boyle","sequence":"additional","affiliation":[{"name":"Section of Pediatric Infectious Diseases, Department of Pediatrics, University of Chicago, Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trudy V.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Daum","sequence":"additional","affiliation":[{"name":"Section of Pediatric Infectious Diseases, Department of Pediatrics, University of Chicago, Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/517478"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08713-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/344326"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0378-1119(96)82965-9","volume":"167","year":"1995","unstructured":"Domanski, T. L., and K. W. Bayles. 1995. Analysis of Staphylococcus aureus genes encoding penicillin-binding protein 4 and an ABC-type transporter. 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