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The\n            <jats:italic>mef<\/jats:italic>\n            (E)-carrying element mega (macrolide efflux genetic assembly; 5,511 bp) was found to be inserted into a Tn\n            <jats:italic>916<\/jats:italic>\n            -like genetic element present in the chromosomes of the two pneumococcal strains. In both strains, mega was integrated at the same site, an open reading frame identical to\n            <jats:italic>orf6<\/jats:italic>\n            of Tn\n            <jats:italic>916<\/jats:italic>\n            . The new composite element, Tn\n            <jats:italic>2009<\/jats:italic>\n            , was about 23.5 kb and, with the exception of the\n            <jats:italic>tet<\/jats:italic>\n            (M)-coding sequence, appeared to be identical in both strains. By sequencing of the junction fragments of Tn\n            <jats:italic>2009<\/jats:italic>\n            at the site of insertion into the chromosome, it was possible to show that (i) the insertion site was identical in the two clinical strains and (ii) the integration of Tn\n            <jats:italic>2009<\/jats:italic>\n            caused a 9.5 kb-deletion in the pneumococcal chromosome. It was not possible to detect the conjugal transfer of Tn\n            <jats:italic>2009<\/jats:italic>\n            to a recipient pneumococcal strain; however, transfer of the whole element by transformation was shown to occur. It is possible to hypothesize that Tn\n            <jats:italic>2009<\/jats:italic>\n            relies on transformation for its spread among clinical strains of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.48.6.2037-2042.2004","type":"journal-article","created":{"date-parts":[[2004,5,21]],"date-time":"2004-05-21T18:38:42Z","timestamp":1085164722000},"page":"2037-2042","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Tn\n            <i>2009<\/i>\n            , a Tn\n            <i>916<\/i>\n            -Like Element Containing\n            <i>mef<\/i>\n            (E) in\n            <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Maria","family":"Del Grosso","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Scotto d'Abusco","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Iannelli","sequence":"additional","affiliation":[{"name":"LAMMB, Dipartimento di Biologia Molecolare, Universita\u0300 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni","family":"Pozzi","sequence":"additional","affiliation":[{"name":"LAMMB, Dipartimento di Biologia Molecolare, Universita\u0300 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.9.2335"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.5.1617-1622.1991"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2072-2081.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04972-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00778.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.01521.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.774-778.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01723.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/315853"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/321001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.2855-2861.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2844-2849.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.7.2147-2150.2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:26:1:81"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2727-2734.2002"},{"key":"e_1_3_2_17_2","first-page":"563","year":"1993","unstructured":"Maslow, J. 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