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However, low-dose long-term therapy with certain macrolides is effective in most patients with DPB. The present study was designed to examine the effects of long-term erythromycin (ERY) therapy by using our established murine model of chronic respiratory<jats:italic>P. aeruginosa<\/jats:italic>infection. ERY or saline was administered from day 80 after intubation with a<jats:italic>P. aeruginosa<\/jats:italic>-precoated tube for the subsequent 10, 20, 40, and 80 days. Bacteriologic and histologic analyses of the murine lungs and electron microscopy of the intubated tube were performed. In the murine model, treatment with ERY for 80 days significantly reduced the number of viable<jats:italic>P. aeruginosa<\/jats:italic>organisms in the lungs (<jats:italic>P<\/jats:italic>&lt; 0.05). The biofilm formed in situ by<jats:italic>P. aeruginosa<\/jats:italic>on the inner wall of the inoculation tube placed into the murine bronchus became significantly thinner after 80 days of ERY treatment. We conclude that the clinical efficacy of macrolides in DPB may be due at least in part to the reduction in<jats:italic>P. aeruginosa<\/jats:italic>biofilm formation.<\/jats:p>","DOI":"10.1128\/aac.48.6.2251-2259.2004","type":"journal-article","created":{"date-parts":[[2004,5,21]],"date-time":"2004-05-21T18:38:42Z","timestamp":1085164722000},"page":"2251-2259","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Effect of Erythromycin on Chronic Respiratory Infection Caused by<i>Pseudomonas aeruginosa<\/i>with Biofilm Formation in an Experimental Murine Model"],"prefix":"10.1128","volume":"48","author":[{"given":"Towako","family":"Nagata","sequence":"first","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mukae","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Kadota","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine, Medical School, Oita University, Oita, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomayoshi","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nagasaki University Hospital, Nagasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misuzu","family":"Kuroki","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Shirai","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsunori","family":"Yanagihara","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Tomono","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiko","family":"Koji","sequence":"additional","affiliation":[{"name":"Department of Histology and Cell Biology, School of Medicine, Nagasaki University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Kohno","sequence":"additional","affiliation":[{"name":"Second Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11081-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg397"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.50.12.1246"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.49.6.531"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1378\/chest.83.1.63","volume":"83","year":"1983","unstructured":"Homma, H., A. 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