{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:28:17Z","timestamp":1790911697892,"version":"4.1.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The effect of the two antibiotics ceftazidime and meropenem on a collection of 46\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            isolates representing clinical and environmental sources across northern Australia was investigated by using a series of in vitro test methods. The susceptibility testing methods used included Kirby-Bauer disk diffusion, Etest MIC, broth microdilution MIC, and a modification of the microdilution method in which\n            <jats:italic>Acanthamoeba<\/jats:italic>\n            cells were added to simulate the effect of a professional phagocytic cell on test outcome. In a semiquantitative validation coculture series, the majority of bacteria were intracellular up to a multiplicity of infection of 10 bacteria to one ameba. The optical density and bacterial count (log\n            <jats:sub>10<\/jats:sub>\n            CFU\/ml) correlated across the range tested (\n            <jats:italic>r<\/jats:italic>\n            <jats:sup>2<\/jats:sup>\n            = 0.77;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). Susceptibility test results were compared against clinical outcomes. The MICs of ceftazidime were consistently higher than those of meropenem by all three methods. The MICs of both agents were significantly higher when\n            <jats:italic>Acanthamoeba<\/jats:italic>\n            trophozoites were added to the broth microdilution method. Conventional and intracellular MIC results were consistent for clinical isolates from the Western Australian outbreak cluster despite the wide variety of clinical outcomes. Further development of the intracellular MIC method is expected to help assess the efficacy of antimicrobial agents on this bacterial species in an intracellular setting.\n          <\/jats:p>","DOI":"10.1128\/aac.48.8.2999-3005.2004","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T18:45:20Z","timestamp":1090608320000},"page":"2999-3005","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Comparison of the Susceptibilities of\n            <i>Burkholderia pseudomallei<\/i>\n            to Meropenem and Ceftazidime by Conventional and Intracellular Methods"],"prefix":"10.1128","volume":"48","author":[{"given":"T. J. J.","family":"Inglis","sequence":"first","affiliation":[{"name":"The Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"The Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Rigby","sequence":"additional","affiliation":[{"name":"Lotteries Confocal Microscopy Centre, Department of Pharmacology, Faculty of Medicine and Dentistry, University of Western Australia, Western Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Norton","sequence":"additional","affiliation":[{"name":"QHPS, Townsville General Hospital, Townsville, Queensland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. J.","family":"Currie","sequence":"additional","affiliation":[{"name":"The Menzies School of Health Research and Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.5.1181"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(99)00060-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/24.3.295"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.12.3243-3248.1992"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899002964"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1681-1686.2000"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4269\/ajtmh.2001.65.76","volume":"65","year":"2001","unstructured":"Inglis, T. J. J., C. L. Golledge, A. Clair, and J. Harvey. 2001. Case report: recovery from persistent septicemic melioidosis. Am. J. Trop. Med.65:76-82.","journal-title":"Am. J. Trop. Med."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3198-3203.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(00)00334-4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.3.782-790.1996"},{"key":"e_1_3_2_12_2","unstructured":"National Committee for Clinical Laboratory Standards. 1993. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A5. National Committee for Clinical Laboratory Standards Villanova Pa."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/520219"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/37.3.611"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg011"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90768-X"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.8.2999-3005.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.48.8.2999-3005.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T18:33:01Z","timestamp":1645468381000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.48.8.2999-3005.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":15,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1128\/AAC.48.8.2999-3005.2004"],"URL":"https:\/\/doi.org\/10.1128\/aac.48.8.2999-3005.2004","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8]]},"assertion":[{"value":"2003-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}