{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:17:30Z","timestamp":1769041050959,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2004,9,1]],"date-time":"2004-09-01T00:00:00Z","timestamp":1093996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extended-spectrum \u03b2-lactamases (ESBLs) in\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            , such as GES-2, which compromises the efficacy of imipenem, tend to be geographically restricted. The CC-to-AA base pair substitution at positions 493 and 494 of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-2<\/jats:sub>\n            -coding region distinguishes this ESBL from\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-1<\/jats:sub>\n            and the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IBC<\/jats:sub>\n            -type genes, making it an ideal target for the development of a novel sequence-specific, peptide nucleic acid (PNA)-based multiplex PCR detection method. By using two primer pairs in conjunction with a PNA probe, this method provided an accurate means of identification of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-2<\/jats:sub>\n            compared to standard PCR and gene sequencing techniques when it was used to test 100\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            clinical isolates as well as previously published, well-described control strains encompassing all presently known genes in the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-IBC<\/jats:sub>\n            ESBL family. This novel method has the potential to be used in large-scale, cost-effective screening programs for specific or geographically restricted ESBLs.\n          <\/jats:p>","DOI":"10.1128\/aac.48.9.3402-3406.2004","type":"journal-article","created":{"date-parts":[[2004,8,24]],"date-time":"2004-08-24T19:12:57Z","timestamp":1093374777000},"page":"3402-3406","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Sequence-Selective Recognition of Extended-Spectrum \u03b2-Lactamase GES-2 by a Competitive, Peptide Nucleic Acid-Based Multiplex PCR Assay"],"prefix":"10.1128","volume":"48","author":[{"given":"Gerhard F.","family":"Weldhagen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Pathology, Faculty of Health Sciences, University of Pretoria, and National Health Laboratory Service, Pretoria, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","first-page":"203","volume":"134","year":"1995","unstructured":"Arlet, G., G. 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