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UV-induced<jats:italic>Aspergillus nidulans<\/jats:italic>mutants resistant to terbinafine have been identified, and we report here the characterization of one such gene. A sib-selected, 6.6-kb genomic DNA fragment encodes a salicylate 1-monooxygenase (<jats:italic>salA<\/jats:italic>), and a fatty acid synthase subunit (<jats:italic>fasC<\/jats:italic>) confers terbinafine resistance upon transformation of a sensitive strain. Subfragments carrying<jats:italic>salA<\/jats:italic>but not<jats:italic>fasC<\/jats:italic>confer terbinafine resistance.<jats:italic>salA<\/jats:italic>is present as a single-copy gene on chromosome VI and encodes a protein of 473 amino acids that is homologous to salicylate 1-monooxygenase, a well-characterized naphthalene-degrading enzyme in bacteria.<jats:italic>salA<\/jats:italic>transcript accumulation analysis showed terbinafine-dependent induction in the wild type and the UV-induced mutant Terb7, as well as overexpression in a strain containing the<jats:italic>salA<\/jats:italic>subgenomic DNA fragment, probably due to the multicopy effect caused by the transformation event. Additional naphthalene degradation enzyme-coding genes are present in fungal genomes, suggesting that resistance could follow degradation of the naphthalene ring contained in terbinafine.<\/jats:p>","DOI":"10.1128\/aac.48.9.3530-3535.2004","type":"journal-article","created":{"date-parts":[[2004,8,24]],"date-time":"2004-08-24T19:12:57Z","timestamp":1093374777000},"page":"3530-3535","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Terbinafine Resistance Mediated by Salicylate 1-Monooxygenase in<i>Aspergillus nidulans<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Marcia A. S.","family":"Graminha","sequence":"first","affiliation":[{"name":"Departamento de Gene\u0301tica, Faculdade de Medicina de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo, Ribeira\u0303o Preto, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleusa M. 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