{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:06:30Z","timestamp":1790838390109,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    CF349 exhibited a complex \u03b2-lactam resistance phenotype, including resistance to amoxicillin and ticarcillin alone and in combination with clavulanate and to some extended-spectrum cephalosporins. The double-disk synergy test was positive. CF349 harbored an 85-kb conjugative plasmid which encoded a \u03b2-lactamase of pI 5.9. The corresponding\n                    <jats:italic>bla<\/jats:italic>\n                    gene was identified by PCR and sequencing as a\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    gene. The deduced protein sequence revealed a new complex mutant of TEM-1 \u03b2-lactamase designated TEM-109 (CMT-5). TEM-109 contained both the substitutions Glu104Lys and Arg164His of the expanded-spectrum \u03b2-lactamase (ESBL) TEM-6 and Met69Leu of the inhibitor-resistant TEM-33 (IRT-5). TEM-109 exhibited hydrolytic activity against ceftazidime similar to that of TEM-6 (\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>cat<\/jats:sub>\n                    , 56 s\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 105 s\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively;\n                    <jats:italic>\n                      K\n                      <jats:sub>m<\/jats:sub>\n                    <\/jats:italic>\n                    values, 226 and 247 \u03bcM, respectively). The 50% inhibitory concentrations of clavulanate and tazobactam (0.13 \u03bcM and 0.27\u2009\u03bcM, respectively) were 5- to 10-fold higher for TEM-109 than for TEM-6 (0.01 and 0.06 \u03bcM, respectively) but were almost 10-fold lower than those for TEM-33. The characterization of this novel CMT, which exhibits a low level of resistance to inhibitors, highlights the emergence of this new ESBL type.\n                  <\/jats:p>","DOI":"10.1128\/aac.49.11.4443-4447.2005","type":"journal-article","created":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T12:37:59Z","timestamp":1130330279000},"page":"4443-4447","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["TEM-109 (CMT-5), a Natural Complex Mutant of TEM-1 \u03b2-Lactamase Combining the Amino Acid Substitutions of TEM-6 and TEM-33 (IRT-5)"],"prefix":"10.1128","volume":"49","author":[{"given":"F.","family":"Robin","sequence":"first","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Delmas","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chanal","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sirot","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sirot","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine, Centre Hospitalo-Universitaire, Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01644127"},{"key":"e_1_3_2_4_2","first-page":"75","volume":"120","year":"1994","unstructured":"Belaaouaj, A., C. 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