{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:04:24Z","timestamp":1789103064960,"version":"build-2803163510"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The rate of occurrence of the extended-spectrum beta-lactamase (ESBL)-producing phenotype among\n            <jats:italic>Escherichia coli<\/jats:italic>\n            isolates in Tel Aviv is 12% (22). The aim of this study was to understand the molecular epidemiology of\n            <jats:italic>E. coli<\/jats:italic>\n            ESBL producers and to identify the ESBL genes carried by them. We studied 20 single-patient ESBL-producing\n            <jats:italic>E. coli<\/jats:italic>\n            clinical isolates. They comprised 11 distinct nonrelated pulsed-field gel electrophoresis (PFGE) genotypes: six isolates belonged to the same PFGE clone, four other clones included two isolates each, and six unrelated clones included only one isolate. All isolates produced various beta-lactamases with pIs ranging from 5.2 to 8.2, varying within similar PFGE clones. The most prevalent ESBL gene was\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            ; 16 isolates carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            and three carried a new ESBL gene designated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-39<\/jats:sub>\n            . Three strains carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            (two\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-12<\/jats:sub>\n            and one\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-5<\/jats:sub>\n            ), and two strains carried inhibitor-resistant ESBL genes,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-33<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-30<\/jats:sub>\n            ; 18 strains carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            and eight strains carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-2<\/jats:sub>\n            . Plasmid mapping and Southern blot analysis with a CTX-M-2 probe demonstrated that\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            is plasmid borne. The wide dissemination of ESBLs among\n            <jats:italic>E. coli<\/jats:italic>\n            isolates in our institution is partly related to clonal spread, but more notably to various plasmid-associated ESBL genes, occurring in multiple clones, wherein the CTX-M gene family appears almost uniformly. We report here a new CTX-M gene, designated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-39<\/jats:sub>\n            , which revealed 99% homology with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-26<\/jats:sub>\n            , with a substitution of arginine for glutamine at position 225.\n          <\/jats:p>","DOI":"10.1128\/aac.49.11.4745-4750.2005","type":"journal-article","created":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T16:37:59Z","timestamp":1130344679000},"page":"4745-4750","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple\n            <i>Escherichia coli<\/i>\n            Clones Isolated in Tel Aviv, Israel"],"prefix":"10.1128","volume":"49","author":[{"given":"Inna","family":"Chmelnitsky","sequence":"first","affiliation":[{"name":"Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azita","family":"Leavitt","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell J.","family":"Schwaber","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiri","family":"Navon-Venezia","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"837","volume":"146","year":"1940","unstructured":"Abraham, E. 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