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The basis of resistance to this drug was investigated by isolating and characterizing capreomycin-resistant strains of\n            <jats:italic>Mycobacterium smegmatis<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            . Colonies resistant to capreomycin were recovered from a library of transposon-mutagenized\n            <jats:italic>M. smegmatis<\/jats:italic>\n            . The transposon insertion site of one mutant was mapped to an open reading frame in the unfinished\n            <jats:italic>M. smegmatis<\/jats:italic>\n            genome corresponding to the\n            <jats:italic>tlyA<\/jats:italic>\n            gene (Rv1694) in the\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            H37Rv genome. In\n            <jats:italic>M. smegmatis<\/jats:italic>\n            spontaneous capreomycin-resistant mutants, the\n            <jats:italic>tlyA<\/jats:italic>\n            gene was disrupted by one of three different naturally occurring insertion elements. Genomic DNAs from pools of transposon mutants of\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            H37Rv were screened by PCR by using primers to the\n            <jats:italic>tlyA<\/jats:italic>\n            gene and the transposon to detect mutants with an insertion in the\n            <jats:italic>tlyA<\/jats:italic>\n            gen\n            <jats:italic>e<\/jats:italic>\n            . One capreomycin-resistant mutant was recovered that contained the transposon inserted at base 644 of the\n            <jats:italic>tlyA<\/jats:italic>\n            gene. Complementation with the wild-type\n            <jats:italic>tlyA<\/jats:italic>\n            gene restored susceptibility to capreomycin in the\n            <jats:italic>M. smegmatis<\/jats:italic>\n            and\n            <jats:italic>M. tuberculosis tlyA<\/jats:italic>\n            transposon mutants. Mutations were found in the\n            <jats:italic>tlyA<\/jats:italic>\n            genes of 28 spontaneous capreomycin-resistant mutants generated from three different\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            strains and in the\n            <jats:italic>tlyA<\/jats:italic>\n            genes of capreomycin-resistant clinical isolates. In in vitro transcription-translation assays, ribosomes from\n            <jats:italic>tlyA<\/jats:italic>\n            mutant but not\n            <jats:italic>tlyA<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            strains resist capreomycin inhibition of transcription-translation. Therefore, TlyA appears to affect the ribosome, and mutation of\n            <jats:italic>tlyA<\/jats:italic>\n            confers capreomycin resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.49.2.571-577.2005","type":"journal-article","created":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T22:04:40Z","timestamp":1106777080000},"page":"571-577","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":205,"title":["Mutation of\n            <i>tlyA<\/i>\n            Confers Capreomycin Resistance in\n            <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"49","author":[{"given":"Courtney E.","family":"Maus","sequence":"first","affiliation":[{"name":"Program in Microbiology and Molecular Genetics, Emory University"},{"name":"Division of TB Elimination, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie B.","family":"Plikaytis","sequence":"additional","affiliation":[{"name":"Division of TB Elimination, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Shinnick","sequence":"additional","affiliation":[{"name":"Division of TB Elimination, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1295"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1159\/000386801","volume":"16","year":"1970","unstructured":"Algeorge, G., and A. 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