{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:13:26Z","timestamp":1778667206341,"version":"3.51.4"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The in vitro susceptibilities of 36 clinical isolates of<jats:italic>Madurella mycetomatis<\/jats:italic>, the prime agent of eumycetoma in Africa, to ketoconazole, itraconazole, fluconazole, voriconazole, amphotericin B, and flucytosine were determined by the Sensititre YeastOne system. This system appeared to be a rapid and easy test, and by use of hyphal suspensions it generated results comparable to those of a modified NCCLS method. After 10 days of incubation, the antifungal activities of ketoconazole (MIC at which 90% of isolates were inhibited [MIC<jats:sub>90<\/jats:sub>], 0.125 \u03bcg\/ml), itraconazole (MIC<jats:sub>90<\/jats:sub>, 0.064 \u03bcg\/ml), and voriconazole (MIC<jats:sub>90<\/jats:sub>, 0.125 \u03bcg\/ml) appeared superior to those of fluconazole (MIC<jats:sub>90<\/jats:sub>, 128 \u03bcg\/ml) and amphotericin B (MIC<jats:sub>90<\/jats:sub>, 1 \u03bcg\/ml), with MICs in the clinically relevant range. All isolates were resistant to flucytosine (all MICs above 64 \u03bcg\/ml). Based on the relatively broad range of MICs obtained for the antifungal agents, routine testing of<jats:italic>M. mycetomatis<\/jats:italic>isolates for susceptibility to antifungal agents seems to be relevant to adequate therapeutic management.<\/jats:p>","DOI":"10.1128\/aac.49.4.1364-1368.2005","type":"journal-article","created":{"date-parts":[[2005,3,25]],"date-time":"2005-03-25T19:23:13Z","timestamp":1111778593000},"page":"1364-1368","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Testing of the In Vitro Susceptibilities of<i>Madurella mycetomatis<\/i>to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2<i>H<\/i>-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method"],"prefix":"10.1128","volume":"49","author":[{"given":"Wendy W. 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