{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:47:28Z","timestamp":1790401648591,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The contribution of integrons to the dissemination of extended-spectrum \u03b2-lactamases (ESBL) was analyzed on all ESBL-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            isolates from 1988 to 2000 at Ramo\u0301n y Cajal Hospital. We studied 133\n            <jats:italic>E. coli<\/jats:italic>\n            pulsed-field gel electrophoresis types: (i) 52 ESBL-producing clinical strains (C-ESBL) (16 TEM, 9 SHV, 21 CTX-M-9, 1 CTX-M-14, and 5 CTX-M-10); (ii) 43 non-ESBL blood clinical strains (C-nESBL); and (iii) 38 non-ESBL fecal isolates from healthy volunteers (V-nESBL). Class 1 integrons were more common among C-ESBL (67%) than among C-nESBL (40%) or V-nESBL (26%) (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001) due to the high number of strains with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>\n              <jats:italic>CTX<\/jats:italic>\n              -\n              <jats:italic>M<\/jats:italic>\n              -\n              <jats:italic>9<\/jats:italic>\n            <\/jats:sub>\n            , which is linked to an In6-like class 1 integron. Without this bias, class 1 integron occurrence would be similar in C-ESBL and C-nESBL groups (47% versus 40%). Occurrence of class 2 integrons was similar among clinical and community isolates (13 to 18%). No isolates contained class 3 integrons. The relatively low rate of class 1 integrons within transferable elements carrying\n            <jats:italic>\n              bla\n              <jats:sub>TEM<\/jats:sub>\n            <\/jats:italic>\n            (23%) or\n            <jats:italic>\n              bla\n              <jats:sub>SHV<\/jats:sub>\n            <\/jats:italic>\n            (33%) and the absence of class 2 integrons in all ESBL transconjugants mirror the assembly of translocative pieces containing\n            <jats:italic>\n              bla\n              <jats:sub>TEM<\/jats:sub>\n            <\/jats:italic>\n            or\n            <jats:italic>\n              bla\n              <jats:sub>SHV<\/jats:sub>\n            <\/jats:italic>\n            on local available transferable elements lacking integrons. The low diversity of class 1 integrons (seven types recovered in all groups) might indicate a wide dissemination of specific genetic elements in which they are located. In our environment, the spread of genetic elements encoding ESBL has no major impact on the dispersion of integrons, nor do integrons have a major impact on the spread of ESBL, except when\n            <jats:italic>\n              bla\n              <jats:sub>ESBL<\/jats:sub>\n            <\/jats:italic>\n            genes are within an integron platform such as\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>\n              <jats:italic>CTX<\/jats:italic>\n              -\n              <jats:italic>M<\/jats:italic>\n              -\n              <jats:italic>9<\/jats:italic>\n            <\/jats:sub>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.49.5.1823-1829.2005","type":"journal-article","created":{"date-parts":[[2005,4,26]],"date-time":"2005-04-26T23:32:47Z","timestamp":1114558367000},"page":"1823-1829","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":155,"title":["Integron Content of Extended-Spectrum-\u03b2-Lactamase-Producing\n            <i>Escherichia coli<\/i>\n            Strains over 12 Years in a Single Hospital in Madrid, Spain"],"prefix":"10.1128","volume":"49","author":[{"given":"Elisabete","family":"Machado","sequence":"first","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Faculdade de Farma\u0301cia, Universidade do Porto, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Canto\u0301n","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Baquero","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan-Carlos","family":"Gala\u0301n","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azucena","family":"Rolla\u0301n","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lui\u0301sa","family":"Peixe","sequence":"additional","affiliation":[{"name":"Faculdade de Farma\u0301cia, Universidade do Porto, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa M.","family":"Coque","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.7.1612"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro909"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.10.3326-3331.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1097\/00001432-200308000-00003","volume":"16","year":"2003","unstructured":"Cant\u00f3n, R., T. 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