{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:48:40Z","timestamp":1792723720094,"version":"4.5.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Endocarditis is the major clinical manifestation of chronic Q fever. Although doxycycline along with hydroxychloroquine remains the mainstay of medical therapy for Q fever endocarditis, there are wide variations in the rapidity of the patient's decline of antibody levels during such therapy. We undertook a retrospective examination of whether there was any correlation between the ratio of serum concentration to MIC of doxycycline and response to treatment in patients with Q fever endocarditis. Included herein are 16 patients from whom\n            <jats:italic>Coxiella burnetii<\/jats:italic>\n            was isolated from cardiac valve materials. Serology and measurement of doxycycline and hydroxychloroquine serum levels were performed and recorded after 1 year of treatment. The MIC of doxycycline for\n            <jats:italic>C. burnetii<\/jats:italic>\n            isolates was determined using the shell vial assay in a real-time quantitative PCR assay. At the completion of a yearlong therapy with doxycycline-hydroxychloroquine, all those that showed a low decline of antibody levels (\n            <jats:italic>n<\/jats:italic>\n            = 6) (i.e., &lt;2-fold decrease in antibody titer to phase I\n            <jats:italic>C. burnetii<\/jats:italic>\n            antigen) had a ratio of serum doxycycline concentration to MIC between 0.5 and 1. In contrast, those having a ratio of \u22651 showed a rapid decline of phase I antibody levels (\n            <jats:italic>n<\/jats:italic>\n            = 9;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). The only patient who died had a serum doxycycline-to-MIC ratio of &lt;0.5, and the isolate of\n            <jats:italic>C. burnetii<\/jats:italic>\n            cultured from this patient was resistant to doxycycline (MIC = 8 \u03bcg\/ml). The ratio of serum doxycycline concentration to MIC should be monitored during the course of therapy in patients with Q fever endocarditis.\n          <\/jats:p>","DOI":"10.1128\/aac.49.7.2673-2676.2005","type":"journal-article","created":{"date-parts":[[2005,6,25]],"date-time":"2005-06-25T02:22:50Z","timestamp":1119666170000},"page":"2673-2676","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Correlation between Ratio of Serum Doxycycline Concentration to MIC and Rapid Decline of Antibody Levels during Treatment of Q Fever Endocarditis"],"prefix":"10.1128","volume":"49","author":[{"given":"Jean-Marc","family":"Rolain","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areen","family":"Boulos","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Noe\u0308lle","family":"Mallet","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.07.007"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1869-1874.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3989-3995.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0002-9343(96)00040-X","volume":"100","year":"1996","unstructured":"Fournier, P. E., J. P. Casalta, G. Habib, T. Messana, and D. Raoult. 1996. Modification of the diagnostic criteria proposed by the Duke Endocarditis Service to permit improved diagnosis of Q fever endocarditis. Am. J. Med.100:629-633.","journal-title":"Am. J. Med."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.7.1823-1834.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.10.2747"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.11.3276-3278.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/324621"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00804-1"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.5.1097"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/340246"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.41.3.1089-1098.1983"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1001\/archinte.159.2.167","volume":"159","year":"1999","unstructured":"Raoult, D., P. Houpikian, H. Tissot Dupont, J. M. Riss, J. Arditi-Djiane, and P. Brouqui. 1999. Treatment of Q fever endocarditis: comparison of two regimens containing doxycycline and ofloxacin or hydroxychloroquine. Arch. Int. Med.159:167-173.","journal-title":"Arch. Int. Med."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.10.2070"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.11.2482-2484.1990"},{"key":"e_1_3_2_18_2","first-page":"1322","volume":"9","year":"2003","unstructured":"Rolain, J. M., M. N. Mallet, and D. Raoult. 2003. Correlation between serum levels of doxycycline and serology evolution in patients treated for Coxiella burnetii endocarditis. J. Infect. Dis.9:1322-1325.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1391-1393.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.301-302.2001"},{"key":"e_1_3_2_21_2","first-page":"375","year":"1981","unstructured":"Spicer, A. J., M. G. Peacock, and J. C. Williams. 1981. Effectiveness of several antibiotics in suppressing chick embryo lethality during experimental infections by Coxiella burnetii,Rickettsia typhi, and R. rickettsii, p. 375-383. In W. Burgdorfer and R. L. Anacker (ed.), Rickettsiae and rickettsial diseases. Academic Press, New York, N.Y.","journal-title":"Rickettsiae and rickettsial diseases"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.1.2.189-196.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/340247"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.49.7.2673-2676.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.49.7.2673-2676.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:06:04Z","timestamp":1645470364000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.49.7.2673-2676.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1128\/AAC.49.7.2673-2676.2005"],"URL":"https:\/\/doi.org\/10.1128\/aac.49.7.2673-2676.2005","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7]]},"assertion":[{"value":"2005-02-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-03-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}