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To study the contribution of rRNA mutations to the emergence of drug resistance in the\n            <jats:italic>Chlamydiaceae<\/jats:italic>\n            , we used the sensitivities of\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            L2 (two\n            <jats:italic>rrn<\/jats:italic>\n            operons) and\n            <jats:italic>Chlamydophila psittaci<\/jats:italic>\n            6BC (one\n            <jats:italic>rrn<\/jats:italic>\n            operon) to the aminoglycoside spectinomycin as a model. Confluent cell monolayers were infected in a plaque assay with about 10\n            <jats:sup>8<\/jats:sup>\n            wild-type infectious particles and then treated with the antibiotic. After a 2-week incubation time, plaques formed by spontaneous spectinomycin-resistant (Spc\n            <jats:sup>r<\/jats:sup>\n            ) mutants appeared with a frequency of 5 \u00d7 10\n            <jats:sup>\u22125<\/jats:sup>\n            for\n            <jats:italic>C. psittaci<\/jats:italic>\n            6BC. No Spc\n            <jats:sup>r<\/jats:sup>\n            mutants were isolated for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            L2, although the frequencies of rifampin resistance were in the same range for both strains (i.e., 10\n            <jats:sup>\u22127<\/jats:sup>\n            ). The risk of emergence of\n            <jats:italic>Chlamydia<\/jats:italic>\n            strains resistant to tetracyclines and macrolides, the ribosomal drugs currently used to treat chlamydial infections, is discussed.\n          <\/jats:p>","DOI":"10.1128\/aac.49.7.2865-2873.2005","type":"journal-article","created":{"date-parts":[[2005,6,25]],"date-time":"2005-06-25T02:22:50Z","timestamp":1119666170000},"page":"2865-2873","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Frequency of Spontaneous Mutations That Confer Antibiotic Resistance in\n            <i>Chlamydia<\/i>\n            spp"],"prefix":"10.1128","volume":"49","author":[{"given":"Rachel","family":"Binet","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, F. 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