{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:44:57Z","timestamp":1792597497058,"version":"4.5.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n The diversity and evolution of the class A OXY \u03b2-lactamase from\n Klebsiella oxytoca<\/jats:italic>\n were investigated and compared to housekeeping gene diversity. The entire\n bla<\/jats:italic>\n OXY<\/jats:sub>\n coding region was sequenced in 18 clinical isolates representative of\nthe four\n K. oxytoca<\/jats:italic>\n \u03b2-lactamase gene groups\n bla<\/jats:italic>\n OXY-1<\/jats:sub>\n to\n bla<\/jats:italic>\n OXY-4<\/jats:sub>\n and of two\nnew groups identified here,\n bla<\/jats:italic>\n OXY-5<\/jats:sub>\n (with four isolates with pI 7.2 and one with pI 7.7) and\n bla<\/jats:italic>\n OXY-6<\/jats:sub>\n (with four isolates with pI 7.75 and three with pI 8.1). Genes\n bla<\/jats:italic>\n OXY-5<\/jats:sub>\n and\n bla<\/jats:italic>\n OXY-6<\/jats:sub>\n showed 99.8% within-group nucleotide similarity but differed from each other by 4.2% and from\n bla<\/jats:italic>\n OXY-1<\/jats:sub>\n , their closest relative, by 2.5% and 2.9%, respectively. Antimicrobial susceptibility to \u03b2-lactams was\nsimilar among OXY groups. Nucleotide sequence diversity of the 16S rRNA (1,454 bp),\n rpoB<\/jats:italic>\n (940 bp),\n gyrA<\/jats:italic>\n (383 bp), and\n gapDH<\/jats:italic>\n (573 bp) genes was in agreement with the \u03b2-lactamase gene phylogeny. Strains with\n bla<\/jats:italic>\n OXY-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXY-2<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXY-3<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXY-4<\/jats:sub>\n , and\n bla<\/jats:italic>\n OXY-6<\/jats:sub>\n genes formed five phylogenetic groups, named KoI, KoII, KoIII, KoIV, and KoVI, respectively. Isolates\nharboring\n bla<\/jats:italic>\n OXY-5<\/jats:sub>\n appeared to represent an emerging lineage within KoI. We estimated that the\n bla<\/jats:italic>\n OXY<\/jats:sub>\n gene has been evolving within\n K. oxytoca<\/jats:italic>\n for approximately 100 million years, using as\ncalibration the 140-million-year estimation of the\n Escherichia coli<\/jats:italic>\n -\n Salmonella enterica<\/jats:italic>\n split. These\nresults show that the\n bla<\/jats:italic>\n OXY<\/jats:sub>\n gene has diversified along\n K. oxytoca<\/jats:italic>\n phylogenetic lines over long periods\nof time without concomitant evolution of the antimicrobial resistance phenotype.\n <\/jats:p>","DOI":"10.1128\/aac.49.8.3453-3462.2005","type":"journal-article","created":{"date-parts":[[2005,7,27]],"date-time":"2005-07-27T10:52:45Z","timestamp":1122461565000},"page":"3453-3462","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Six Groups of the OXY \u03b2-Lactamase Evolved over Millions of Years in\n Klebsiella oxytoca<\/i>"],"prefix":"10.1128","volume":"49","author":[{"given":"Cindy","family":"Fevre","sequence":"first","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Jbel","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Passet","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois-Xavier","family":"Weill","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick A. D.","family":"Grimont","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Brisse","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.1.63"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1190-1198.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050014"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-3-915"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a025606"},{"key":"e_1_3_2_7_2","unstructured":"Comit\u00e9\nde l'Antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de\nMicrobiologie. 2004. 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Lett."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.850"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.460"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1365"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.454"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/40.4.533"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2922-2928.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/ijs.0.02408-0","volume":"53","year":"2003","unstructured":"Granier, S. A., L. Plaisance, V. Leflon-Guibout, E. Lagier, S. Morand, F. W. Goldstein, and M. H. Nicolas-Chanoine. 2003 . Recognition of two genetic groups in the Klebsiella oxytoca taxon on the basis of chromosomal beta-lactamase and housekeeping gene sequences as well as ERIC-1R PCR typing. Int. J Syst. Evol. 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