{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:15:44Z","timestamp":1791033344475,"version":"4.1.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The acetohydroxyacid synthase (AHAS) of\n            <jats:italic>Brucella suis<\/jats:italic>\n            can be effectively targeted by the sulfonylureas chlorimuron ethyl and metsulfuron methyl. Growth in minimal medium was inhibited, and multiplication in human macrophages was totally abolished with 100 \u03bcM of sulfonylureas. Metsulfuron methyl-resistant mutants showed reduced viability in macrophages and reduced AHAS activity.\n          <\/jats:p>","DOI":"10.1128\/aac.49.9.3922-3925.2005","type":"journal-article","created":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T22:07:52Z","timestamp":1125094072000},"page":"3922-3925","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Targeting of the Virulence Factor Acetohydroxyacid Synthase by Sulfonylureas Results in Inhibition of Intramacrophagic Multiplication of\n            <i>Brucella suis<\/i>"],"prefix":"10.1128","volume":"49","author":[{"given":"Rose-Anne","family":"Boigegrain","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, Pl. E. Bataillon, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, Pl. E. Bataillon, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Ko\u0308hler","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, Pl. E. Bataillon, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1794-1799.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.56.2.174"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3270161"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00963-7"},{"key":"e_1_3_2_7_2","first-page":"1","volume":"33","year":"2000","unstructured":"Duggleby, R. G., and S. S. Pang. 2000. Acetohydroxyacid synthase. J. Biochem. Mol. Biol.33:1-36.","journal-title":"J. Biochem. Mol. Biol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90393-N"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.59.6.777-782.1950"},{"key":"e_1_3_2_10_2","first-page":"475","volume":"42","year":"1998","unstructured":"Grandoni, J. A., P. T. Marta, and J. V. Schloss. 1998. Inhibitors of branched-chain amino acid biosynthesis as potential antituberculosis agents. J. Antimicrob. Chemother.42:475-482.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3270891"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232454299"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00078-7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi983013m"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"7639","DOI":"10.1074\/jbc.M211648200","volume":"278","year":"2003","unstructured":"Pang, S. S., L. W. Guddat, and R. G. Duggleby. 2003. Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. J. Biol. Chem.278:7639-7644.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192319099"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9605604"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/35021219"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.49.9.3922-3925.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.49.9.3922-3925.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T18:51:12Z","timestamp":1645469472000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.49.9.3922-3925.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9]]},"references-count":17,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["10.1128\/AAC.49.9.3922-3925.2005"],"URL":"https:\/\/doi.org\/10.1128\/aac.49.9.3922-3925.2005","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9]]},"assertion":[{"value":"2005-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}