{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:46:01Z","timestamp":1792572361925,"version":"4.5.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Streptococcus agalactiae<\/jats:italic>\n (group B streptococcus [GBS]) is the leading cause of neonatal and maternal sepsis. Penicillin is recommended for intrapartum prophylaxis, but erythromycin or clindamycin is used for penicillin-allergic carriers. Antibiotic resistance (AR) has increased recently and needs to be monitored. We have developed a multiplex PCR-based reverse line blot (mPCR\/RLB) hybridization assay to detect, simultaneously, seven genes encoding AR\u2014\n erm<\/jats:italic>\n (A\/TR),\n erm<\/jats:italic>\n (B),\n mef<\/jats:italic>\n (A\/E),\n tet<\/jats:italic>\n (M),\n tet<\/jats:italic>\n (O),\n aphA-3<\/jats:italic>\n , and\n aad<\/jats:italic>\n -\n 6<\/jats:italic>\n \u2014and two AR-related genes,\n int<\/jats:italic>\n -Tn and\n mreA<\/jats:italic>\n . We tested 512 GBS isolates from Asia and Australasia and compared mPCR\/RLB with antibiotic susceptibility phenotype or single-gene PCR. Phenotypic resistance to tetracycline was identified in 450 (88%) isolates, of which 442 had\n tet<\/jats:italic>\n (M) (93%) and\/or\n tet<\/jats:italic>\n (O) (6%). Of 67 (13%) erythromycin-resistant isolates, 18 were susceptible to clindamycin, i.e., had the M phenotype, encoded by\n mef<\/jats:italic>\n (A\/E); 39 had constitutive (cMLS\n B<\/jats:sub>\n ) and 10 inducible clindamycin resistance, and of these, 34 contained\n erm<\/jats:italic>\n (B) and 12\n erm<\/jats:italic>\n (A\/TR). Of four additional isolates with\n mef<\/jats:italic>\n (A\/E), three contained\n erm<\/jats:italic>\n (B) with cMLS\n B<\/jats:sub>\n and one was erythromycin susceptible. Of 61 (12%) clindamycin-resistant isolates, 20 were susceptible to erythromycin and two had intermediate resistance. Based on sequencing, 21 of 22 isolates with\n mef<\/jats:italic>\n had\n mef<\/jats:italic>\n (E), and 8 of 353 with\n int<\/jats:italic>\n -Tn had an atypical sequence. Several AR genes,\n erm<\/jats:italic>\n (B),\n tet<\/jats:italic>\n (O),\n aphA-3<\/jats:italic>\n ,\n aad<\/jats:italic>\n -\n 6<\/jats:italic>\n , and\n mef<\/jats:italic>\n (A\/E), were significantly more common among Asian than Australasian isolates, and there were significant differences in distribution of AR genes between GBS serotypes. Our mPCR\/RLB assay is simple, rapid, and suitable for surveillance of antibiotic resistance in GBS.\n <\/jats:p>","DOI":"10.1128\/aac.50.1.204-209.2006","type":"journal-article","created":{"date-parts":[[2005,12,23]],"date-time":"2005-12-23T10:07:51Z","timestamp":1135332471000},"page":"204-209","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Simultaneous Detection of Nine Antibiotic Resistance-Related Genes in\n Streptococcus agalactiae<\/i>\n Using Multiplex PCR and Reverse Line Blot Hybridization Assay"],"prefix":"10.1128","volume":"50","author":[{"given":"Xianyu","family":"Zeng","sequence":"first","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia"},{"name":"Department of Dermatology, Wuhan First Hospital, Wuhan, Hubei Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanrong","family":"Kong","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia"},{"name":"Department of Dermatology, Wuhan First Hospital, Wuhan, Hubei Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archie","family":"Darbar","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendolyn L.","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1410-1412.2004"},{"key":"e_1_3_2_3_2","first-page":"903","volume":"71","year":"2005","unstructured":"Apgar, B. 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