{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:44:26Z","timestamp":1762865066306},"reference-count":55,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Diagnostic PCR assays were developed to track common genetic determinants of oxacillin resistance as well as resistance to classical tetracyclines in\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates from the recently completed worldwide phase 3 clinical trials of tigecycline. A total of 503 unique\n            <jats:italic>S. aureus<\/jats:italic>\n            strains isolated from complicated skin and skin structure infections were analyzed. The\n            <jats:italic>mecA<\/jats:italic>\n            gene was amplified from 120 strains (23.9%) determined to be resistant to oxacillin (MICs \u2265 4 \u03bcg\/ml). The prevalence of the\n            <jats:italic>mecA<\/jats:italic>\n            gene was found to vary regionally from 6.5% to 50.9% among isolates originating in Eastern Europe and North America, respectively. The presence of a tetracycline resistance determinant,\n            <jats:italic>tet<\/jats:italic>\n            (M) or\n            <jats:italic>tet<\/jats:italic>\n            (K), among methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (MRSA) isolates also varied regionally, with a range of 11.9% to 46.2% among isolates tested from North America and Eastern Europe, respectively. The occurrence of a tetracycline resistance marker in methicillin-susceptible\n            <jats:italic>S. aureus<\/jats:italic>\n            (MSSA) strains varied from 2.5 to 16.1% among the isolates tested across the regions of study. The presence of\n            <jats:italic>tet<\/jats:italic>\n            (M) or\n            <jats:italic>tet<\/jats:italic>\n            (K) had no discernible effect on the tigecycline MICs for either MRSA or MSSA strains, which is consistent with the ability of the glycylcyclines to retain activity in the presence of both the ribosomal protection and efflux mechanisms of resistance to the tetracyclines.\n          <\/jats:p>","DOI":"10.1128\/aac.50.2.505-510.2006","type":"journal-article","created":{"date-parts":[[2006,1,25]],"date-time":"2006-01-25T21:53:41Z","timestamp":1138226021000},"page":"505-510","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Diagnostic PCR Analysis of the Occurrence of Methicillin and Tetracycline Resistance Genes among\n            <i>Staphylococcus aureus<\/i>\n            Isolates from Phase 3 Clinical Trials of Tigecycline for Complicated Skin and Skin Structure Infections"],"prefix":"10.1128","volume":"50","author":[{"given":"C. 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