{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:02:15Z","timestamp":1790838135816,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nine\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and 5\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    clinical isolates resistant to various cephalosporins and cephamycins were identified in a Japanese general hospital between 1995 and 1997. All nine\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates and one\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolate carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-9<\/jats:sub>\n                    , while the other four\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates harbored a variant of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-9<\/jats:sub>\n                    , namely,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-19<\/jats:sub>\n                    . The pulsed-field gel electrophoresis patterns of the nine CMY-9-producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were almost identical, suggesting their clonal relatedness, while those of the five\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates were divergent. Plasmid profiles, Southern hybridization, and conjugation assays revealed that the genes for the CMY-9 and the CMY-19 \u03b2-lactamases were located on very similar conjugative plasmids in\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    . The genetic environment of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-19<\/jats:sub>\n                    was identical to that of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-9<\/jats:sub>\n                    . A single amino acid substitution, I292S, adjacent to the H-10 helix region was observed between CMY-9 and CMY-19. This substitution was suggested to be responsible for the expansion of the hydrolyzing activity against several broad-spectrum cephalosporins, and this finding was consistent with the kinetic parameters determined with purified enzymes. These findings suggest that the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-19<\/jats:sub>\n                    genes found in the four\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates might have originated from\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-9<\/jats:sub>\n                    gene following a point mutation and dispersed among genetically different\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates via a large transferable plasmid.\n                  <\/jats:p>","DOI":"10.1128\/aac.50.2.534-541.2006","type":"journal-article","created":{"date-parts":[[2006,1,25]],"date-time":"2006-01-25T16:53:41Z","timestamp":1138208021000},"page":"534-541","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Horizontal Transfer of\n                    <i>bla<\/i>\n                    <sub>CMY<\/sub>\n                    -Bearing Plasmids among Clinical\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolates and Emergence of Cefepime-Hydrolyzing CMY-19"],"prefix":"10.1128","volume":"50","author":[{"given":"Jun-ichi","family":"Wachino","sequence":"first","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"},{"name":"Department of Bacteriology and Bacterial Infection Control, Gunma University Graduate School of Medicine, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kurokawa","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satowa","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunikazu","family":"Yamane","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Shibata","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouji","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyoshi","family":"Ike","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Bacterial Infection Control, Gunma University Graduate School of Medicine, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshichika","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 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