{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:12:34Z","timestamp":1789863154858,"version":"4.0.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We carried out a study for the nonculture detection of susceptibility of\n            <jats:italic>Neisseria meningitis<\/jats:italic>\n            to penicillin G in three laboratories of the European Monitoring Group on Meningococci (EMGM). Thirteen clinical samples (cerebrospinal fluids) and corresponding bacterial isolates from 13 cases of invasive meningococcal infection were distributed to the three laboratories. The MICs of penicillin G were determined for the isolates. Each laboratory used an \u201cin-house\u201d PCR-based method to determine alterations to the\n            <jats:italic>penA<\/jats:italic>\n            gene, which is associated with a reduced susceptibility to penicillin G. Nucleotide sequences from the 3\u2032 end of the\n            <jats:italic>penA<\/jats:italic>\n            gene were also determined. We observed a good correlation between genotyping of\n            <jats:italic>penA<\/jats:italic>\n            and the phenotypic determination (MIC) of susceptibility to penicillin G. The results obtained by the three methods for\n            <jats:italic>penA<\/jats:italic>\n            in the samples correlated very well with those obtained in bacterial isolates and with sequence data. The kappa coefficient that was used to estimate the level of agreement between genotypic results varied between 0.65 and 1, indicating a good agreement. This suggests that genotyping can predict susceptibility of\n            <jats:italic>N. meningitidis<\/jats:italic>\n            to penicillin G. These data strongly suggest that genotyping of\n            <jats:italic>penA<\/jats:italic>\n            should be used to determine meningococcal susceptibility to penicillin G in culture-negative cases. Although the nucleotide sequence of\n            <jats:italic>penA<\/jats:italic>\n            may be the gold standard in genotyping of\n            <jats:italic>penA<\/jats:italic>\n            , the less expensive PCR-based approach reported in this study may be quicker when a large number of isolates and clinical samples need to be tested.\n          <\/jats:p>","DOI":"10.1128\/aac.50.3.887-892.2006","type":"journal-article","created":{"date-parts":[[2006,2,22]],"date-time":"2006-02-22T07:32:35Z","timestamp":1140593555000},"page":"887-892","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Interlaboratory Comparison of PCR-Based Methods for Detection of Penicillin G Susceptibility in\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"50","author":[{"given":"Muhamed-Kheir","family":"Taha","sequence":"first","affiliation":[{"name":"Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Leticia","family":"Zarantonelli","sequence":"additional","affiliation":[{"name":"Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Neri","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roci\u0301o","family":"Enriquez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisserias, National Center for Microbiology, National Institute of Health Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio A.","family":"Va\u0301zquez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisserias, National Center for Microbiology, National Institute of Health Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Stefanelli","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90045-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3625-3628.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304607200"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/377739"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.285"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.19.2.316"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.10.3336-3338.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.6.1256"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.305.6846.143"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/001316446002000104"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki048"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199809243391302"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197708252970802"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1174-1179.1995"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05371-0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/35006655"},{"key":"e_1_3_2_19_2","first-page":"54","volume":"336","year":"1990","unstructured":"Saez Nieto, J. A., J. A. Vazquez, and C. Marcos. 1990. Meningococci moderately resistant to penicillin. 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