{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:25:20Z","timestamp":1761895520579},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1975,8,1]],"date-time":"1975-08-01T00:00:00Z","timestamp":176083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1975,8]]},"abstract":"<jats:p>\n            The addition of dialuric acid (a superoxide-generating drug) to a suspension of resting human neutrophils resulted in a stimulation of cellular hexose monophosphate shunt activity. Measurement of oxygen consumption demonstrated a rapid rate of oxygen uptake by the drug alone in aqueous solution. The subsequent addition of catalase (but not of superoxide dismutase) resulted in a substantial release of oxygen, indicating that H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            was accumulating in the media. The generation of O\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            by the drug was verified by measuring the rate of reduction of cytochrome\n            <jats:italic>c<\/jats:italic>\n            by the drug in the presence and absence of authentic superoxide dismutase. The growth of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            B and\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            was inhibited in vitro by addition of the drug to a suitable culture media; the degree of inhibition was dose dependent. This inhibition of bacterial growth could be completely reversed by the addition of as little as 5 \u03bcg of purified catalase, but was not affected by concentrations of superoxide dismutase that were 2,000-fold higher. These results suggest that the dialuric acid-induced stimulation of hexose monophosphate shunt activity in neutrophils is due to accumulated H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            . Further, the contribution of superoxide to the bactericidal activity towards\n            <jats:italic>E. coli<\/jats:italic>\n            and\n            <jats:italic>S. aureus<\/jats:italic>\n            is probably minimal compared with that of H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.8.2.146","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"146-153","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Bactericidal Activity of Superoxide Anion and of Hydrogen Peroxide: Investigations Employing Dialuric Acid, a Superoxide-Generating Drug"],"prefix":"10.1128","volume":"8","author":[{"given":"Lawrence R.","family":"DeChatelet","sequence":"first","affiliation":[{"name":"Department of Biochemistry The Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela S.","family":"Shirley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip R.","family":"Goodson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"McCall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.8.2.146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.8.2.146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T13:51:35Z","timestamp":1645451495000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.8.2.146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,8]]}},"alternative-id":["10.1128\/AAC.8.2.146"],"URL":"https:\/\/doi.org\/10.1128\/aac.8.2.146","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,8]]},"assertion":[{"value":"1975-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}