{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:40:36Z","timestamp":1783467636299,"version":"3.55.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100007197","name":"HHS | U.S. Public Health Service","doi-asserted-by":"publisher","award":["AI057472"],"award-info":[{"award-number":["AI057472"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>\n            Organisms of the\n            <jats:named-content content-type=\"genus-species\">Bacillus cereus<\/jats:named-content>\n            <jats:italic>sensu lato<\/jats:italic>\n            lineage are ubiquitous in the environment and are responsible for toxin-mediated infections ranging from severe food poisoning (\n            <jats:named-content content-type=\"genus-species\">B. cereus<\/jats:named-content>\n            <jats:italic>sensu stricto<\/jats:italic>\n            ) to anthrax (\n            <jats:named-content content-type=\"genus-species\">Bacillus anthracis<\/jats:named-content>\n            ). The increasing incidence of many of these infections, combined with the specter of antibiotic resistance, has created a need for novel antimicrobials with potent activity, including bacteriophages (or phages) and phage-encoded products (i.e., endolysins). In this study, we describe a broadly infective phage, Bcp1, and its encoded endolysin, PlyB, which exhibited a rapidly bacteriolytic effect against all\n            <jats:named-content content-type=\"genus-species\">B. cereus<\/jats:named-content>\n            <jats:italic>sensu lato<\/jats:italic>\n            isolates tested with no evidence of evolving resistance. Importantly, PlyB was highly efficacious in a mouse model of lethal bacteremia with\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            . Both the Bcp1 phage and the PlyB endolysin represent novel mechanisms of action compared to antibiotics, with potential applications to address the evolving problem of antimicrobial resistance.\n          <\/jats:p>","DOI":"10.1128\/aem.00003-19","type":"journal-article","created":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T11:07:50Z","timestamp":1551784070000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["The PlyB Endolysin of Bacteriophage vB_BanS_Bcp1 Exhibits Broad-Spectrum Bactericidal Activity against\n            <i>Bacillus cereus Sensu Lato<\/i>\n            Isolates"],"prefix":"10.1128","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4324-573X","authenticated-orcid":false,"given":"Raymond","family":"Schuch","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York, 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