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Production was achieved by systematically engineering the metabolism of this strain as follows: (i) deletion of its inherent 3MP dioxygenase-encoding gene (\n            <jats:italic>mdo<\/jats:italic>\n            ), (ii) introduction of the\n            <jats:italic>buk-ptb<\/jats:italic>\n            operon (genes encoding the butyrate kinase, Buk, and the phosphotransbutyrylase, Ptb, from\n            <jats:named-content content-type=\"genus-species\">Clostridium acetobutylicum<\/jats:named-content>\n            ), and (iii) overexpression of its own polyhydroxyalkanoate synthase (\n            <jats:italic>\n              phaC\n              <jats:sub>Am<\/jats:sub>\n            <\/jats:italic>\n            ). These measures yielded the potent PMP production strain\n            <jats:named-content content-type=\"genus-species\">A. mimigardefordensis<\/jats:named-content>\n            strain SHX22. The deletion of\n            <jats:italic>mdo<\/jats:italic>\n            was required for adequate synthesis of PMP due to the resulting accumulation of 3MP during utilization of DTDP. Overexpression of the plasmid-borne\n            <jats:italic>buk-ptb<\/jats:italic>\n            operon caused a severe growth repression. This effect was overcome by inserting this operon into the genome. Polyhydroxyalkanoate (PHA) synthases from different origins were compared. The native PHA synthase of\n            <jats:named-content content-type=\"genus-species\">A. mimigardefordensis<\/jats:named-content>\n            (\n            <jats:italic>\n              phaC\n              <jats:sub>Am<\/jats:sub>\n            <\/jats:italic>\n            ) was obviously the best choice to establish homopolythioester production in this strain. In addition, the cultivation conditions, including an appropriate provision of the carbon source, were further optimized to enhance PMP production. The engineered strain accumulated PMP up to approximately 25% (wt\/wt) of the cell dry weight when cultivated in mineral salts medium containing glycerol as the carbon source in addition to DTDP as the sulfur-providing precursor. According to our knowledge, this is the first report of PMP homopolymer production by a metabolically engineered bacterium using DTDP, which is nontoxic, as the precursor substrate.\n          <\/jats:p>","DOI":"10.1128\/aem.00007-12","type":"journal-article","created":{"date-parts":[[2012,2,18]],"date-time":"2012-02-18T04:54:54Z","timestamp":1329540894000},"page":"3286-3297","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Employing a Recombinant Strain of Advenella mimigardefordensis for Biotechnical Production of Homopolythioesters from 3,3\u2032-Dithiodipropionic Acid"],"prefix":"10.1128","volume":"78","author":[{"given":"Yongzhen","family":"Xia","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Molekulare Mikrobiologie und Biotechnologie (IMMB) Westf\u00e4lische Wilhelms-Universit\u00e4t, M\u00fcnster, Germany"},{"name":"State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Hendrik","family":"W\u00fcbbeler","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekulare Mikrobiologie und Biotechnologie (IMMB) Westf\u00e4lische Wilhelms-Universit\u00e4t, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingsheng","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Steinb\u00fcchel","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekulare Mikrobiologie und Biotechnologie (IMMB) Westf\u00e4lische Wilhelms-Universit\u00e4t, M\u00fcnster, Germany"},{"name":"King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01997-10"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.8.1977-1982.1988"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M806762200"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1099\/ijs.0.63267-0","article-title":"Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples","volume":"55","author":"Coenye T","year":"2005","unstructured":"CoenyeT . 2005. 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