{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:54:27Z","timestamp":1789102467840,"version":"build-2803163510"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toxigenic\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            , the causative agent of the epidemic diarrheal disease cholera, interacts with diverse environmental bacteriophages. These interactions promote genetic diversity or cause selective enrichment of phage-resistant bacterial clones. To identify bacterial genes involved in mediating the phage-resistant phenotype, we screened a transposon insertion library of\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1 El Tor biotype strain C6706 to identify mutants showing altered susceptibility to a panel of phages isolated from surface waters in Bangladesh. Mutants with insertion in\n            <jats:italic>cyaA<\/jats:italic>\n            or\n            <jats:italic>crp<\/jats:italic>\n            genes encoding adenylate cyclase or cyclic AMP (cAMP) receptor protein (CRP), respectively, were susceptible to a phage designated JSF9 to which the parent strain was completely resistant. Application of the\n            <jats:italic>cyaA<\/jats:italic>\n            mutant as an indicator strain in environmental phage monitoring enhanced phage detection, and we identified 3 additional phages to which the parent strain was resistant. Incorporation of the\n            <jats:italic>cyaA<\/jats:italic>\n            or\n            <jats:italic>crp<\/jats:italic>\n            mutations into other\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1 strains caused similar alterations in their phage susceptibility patterns, and the susceptibility correlated with the ability of the bacteria to adsorb these phages. Our results suggest that cAMP-CRP-mediated downregulation of phage adsorption may contribute to a mechanism for the\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1 strains to survive predation by multiple environmental phages. Furthermore, the\n            <jats:italic>cyaA<\/jats:italic>\n            or\n            <jats:italic>crp<\/jats:italic>\n            mutant strains may be used as suitable indicators in monitoring cholera phages in the water.\n          <\/jats:p>","DOI":"10.1128\/aem.00008-10","type":"journal-article","created":{"date-parts":[[2010,5,15]],"date-time":"2010-05-15T02:01:39Z","timestamp":1273888899000},"page":"4233-4240","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["The Cyclic AMP (cAMP)-cAMP Receptor Protein Signaling System Mediates Resistance of\n            <i>Vibrio cholerae<\/i>\n            O1 Strains to Multiple Environmental Bacteriophages"],"prefix":"10.1128","volume":"76","author":[{"given":"M. Shamim Hasan","family":"Zahid","sequence":"first","affiliation":[{"name":"Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. M. Zaved","family":"Waise","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kamruzzaman","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amar N.","family":"Ghosh","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Balakrish","family":"Nair","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Mekalanos","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah M.","family":"Faruque","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"214","volume":"4","year":"1987","unstructured":"Ackermann, H.-W. 1987. Bacteriophage taxonomy. Microbiol. Sci.4:214-218.","journal-title":"Microbiol. 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