{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T21:14:41Z","timestamp":1780953281967,"version":"3.54.1"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A significant percentage of the human population is exposed to high levels of naturally occurring airborne dusts. Although the link between airborne particulate inhalation and a variety of respiratory diseases has long been established, little is known about the pathogenic role of the microbial component of the dust. In this study, we applied highly multiplexed PCR and a high-density resequencing microarray (RPM-TEI version 1.0) to screen samples of fine topsoil particles and airborne dust collected in 19 locations in Iraq and Kuwait for the presence of a broad range of human pathogens. The results indicated the presence of potential human pathogens, including\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Clostridium perfringens<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Bacillus<\/jats:named-content>\n            . The presence of\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            , a highly infectious potential biowarfare agent, was confirmed and detected in additional samples by use of a more sensitive technique (real-time PCR), indicating a high prevalence of this organism in the analyzed samples. The detection of potentially viable pathogens in breathable dusts from arid regions of Iraq and Kuwait underscores the importance of further study of these environments.\n          <\/jats:p>","DOI":"10.1128\/aem.00021-11","type":"journal-article","created":{"date-parts":[[2011,5,14]],"date-time":"2011-05-14T02:27:10Z","timestamp":1305340030000},"page":"4285-4292","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Application of a Broad-Range Resequencing Array for Detection of Pathogens in Desert Dust Samples from Kuwait and Iraq"],"prefix":"10.1128","volume":"77","author":[{"given":"Tomasz A.","family":"Leski","sequence":"first","affiliation":[{"name":"Center for Bio\/Molecular Science &amp; Engineering, Code 6900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony P.","family":"Malanoski","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science &amp; Engineering, Code 6900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Gregory","sequence":"additional","affiliation":[{"name":"Chemistry Division, Code 6100, Naval Research Laboratory, Washington, DC 20375"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baochuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science &amp; Engineering, Code 6900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Stenger","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science &amp; Engineering, Code 6900"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1109\/8.1082"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00724-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.050314"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/507539"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2007.00012.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0110-25"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007365919013"},{"key":"e_1_3_2_10_2","first-page":"34","article-title":"The microbiological analysis of Saharan dust and its association with asthma in Barbados","volume":"47","author":"Blades E.","year":"1998","unstructured":"BladesE. NaiduR. MathisonG.. 1998. The microbiological analysis of Saharan dust and its association with asthma in Barbados. West Indian Med. J. 47:34.","journal-title":"West Indian Med. J."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608255104"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0195941700061762"},{"key":"e_1_3_2_13_2","first-page":"857","article-title":"Severe acute pneumonitis among deployed U.S. military personnel\u2014Southwest Asia, March-August 2003","volume":"52","author":"Centers for Disease Control and Prevention","year":"2003","unstructured":"Centers for Disease Control and Prevention. 2003. Severe acute pneumonitis among deployed U.S. military personnel\u2014Southwest Asia, March-August 2003. MMBR Morb. Mortal. Wkly. Rep. 52:857\u2013859.","journal-title":"MMBR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.052522"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/165.9.iv"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:103:1:001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.7205\/MILMED.173.10.949"},{"key":"e_1_3_2_18_2","first-page":"9","article-title":"Saharan dust\u2014a carrier of persistent organic pollutants, metals and microbes to the Caribbean?","volume":"54","author":"Garrison V. H.","year":"2006","unstructured":"GarrisonV. H.. 2006. Saharan dust\u2014a carrier of persistent organic pollutants, metals and microbes to the Caribbean? Rev. Biol. Trop. 54:9\u201321.","journal-title":"Rev. Biol. Trop."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/1352-2310(95)00432-7"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2008.07.007"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-200206000-00003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00039-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-004-0120-8"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1308.070218"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.71.9.694"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9260(74)80135-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2009.01.004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01228-07"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2006.07.004"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00042-10"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-2"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/157.9.452"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/158.7.A6a"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006569"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1086\/507639"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2004.09.015"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000419"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01870-06"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.4337206"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-006-9025-2"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl565"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2006.00132.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atmosenv.2003.10.043"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07304-X"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.46.5.341"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1159\/000195715"},{"key":"e_1_3_2_48_2","first-page":"230","article-title":"Pathogenic rapidly growing Mycobacterium manitobense in the environment of Agra, north India","volume":"126","author":"Parashar D.","year":"2007","unstructured":"ParasharD. DasR. SharmaV. D. ChauhanD. S. KatochV. M.. 2007. Pathogenic rapidly growing Mycobacterium manitobense in the environment of Agra, north India. Indian J. Med. Res. 126:230\u2013232.","journal-title":"Indian J. Med. Res."},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2005.00728.x"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.10.005"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.2307\/4012776"},{"key":"e_1_3_2_52_2","first-page":"527","article-title":"Deposition of siliceous dust in the lungs of the inhabitants of the Saharan regions","volume":"5","author":"Policard A.","year":"1952","unstructured":"PolicardA. ColletA.. 1952. Deposition of siliceous dust in the lungs of the inhabitants of the Saharan regions. AMA Arch. Ind. Hyg. Occup. Med. 5:527\u2013534.","journal-title":"AMA Arch. Ind. Hyg. Occup. Med."},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.3.193"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-10"},{"key":"e_1_3_2_55_2","first-page":"825","article-title":"Nonoccupational pneumoconiosis at high-altitude villages in Central Ladakh","volume":"48","author":"Saiyed H. N.","year":"1991","unstructured":"SaiyedH. N.. 1991. Nonoccupational pneumoconiosis at high-altitude villages in Central Ladakh. Br. J. Ind. Med. 48:825\u2013829.","journal-title":"Br. J. Ind. Med."},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.087551.108"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1029\/2000GL011599"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.24.2997"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0020006"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00486.x"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.319-354.2003"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/1467-8306.9302003"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02070.x"},{"key":"e_1_3_2_64_2","first-page":"2669","volume-title":"Principles and practice of infectious diseases","author":"Young E. J","year":"2005","unstructured":"YoungE. J. 2005. Brucella species, p. 2669\u20132674. In MandellG. L. BennettJ. E. DolinR. (ed.), Principles and practice of infectious diseases. Elsevier, Philadelphia, PA."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00021-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00021-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T21:12:56Z","timestamp":1645650776000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00021-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":63,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1128\/AEM.00021-11"],"URL":"https:\/\/doi.org\/10.1128\/aem.00021-11","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2011-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}