{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:11:03Z","timestamp":1790489463934,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Research and Development Plan of China","award":["2016YFC1200500"],"award-info":[{"award-number":["2016YFC1200500"]}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI129819"],"award-info":[{"award-number":["R01AI129819"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31472039"],"award-info":[{"award-number":["31472039"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601793"],"award-info":[{"award-number":["81601793"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2018,5,15]]},"abstract":"ABSTRACT<\/jats:title>\n \n Ticks are important disease vectors, as they transmit a variety of human and animal pathogens worldwide. Symbionts that coevolved with ticks confer crucial benefits to their host in nutrition metabolism, fecundity, and vector competence. Although over 100 tick species have been identified in China, general information on tick symbiosis is limited. Here, we visualized the tissue distribution of\n Coxiella<\/jats:named-content>\n sp. and\n Rickettsia<\/jats:named-content>\n sp. in lab-reared\n Haemaphysalis longicornis<\/jats:named-content>\n and\n Rhipicephalus haemaphysaloides<\/jats:named-content>\n by fluorescent\n in situ<\/jats:italic>\n hybridization. We found that\n Coxiella<\/jats:named-content>\n sp. colonized exclusively the Malpighian tubules and ovaries of\n H. longicornis<\/jats:named-content>\n , while\n Rickettsia<\/jats:named-content>\n sp. additionally colonized the midgut of\n R. haemaphysaloides<\/jats:named-content>\n . We also investigated the population structure of microbiota in\n Dermacentor silvarum<\/jats:named-content>\n ticks collected from Inner Mongolia, China, and found that\n Coxiella<\/jats:named-content>\n ,\n Rickettsia<\/jats:named-content>\n , and\n Pseudomonas<\/jats:named-content>\n are the three dominant genera. No significant difference in microbiota composition was found between male and female\n D. silvarum<\/jats:named-content>\n ticks. We again analyzed the tissue localization of\n Coxiella<\/jats:named-content>\n sp. and\n Rickettsia<\/jats:named-content>\n sp. and found that they displayed tissue tropisms similar to those in\n R. haemaphysaloides<\/jats:named-content>\n , except that\n Rickettsia<\/jats:named-content>\n sp. colonized the nuclei of spermatids instead of ovaries in\n D. silvarum<\/jats:named-content>\n . Altogether, our results suggest that\n Coxiella<\/jats:named-content>\n sp. and\n Rickettsia<\/jats:named-content>\n sp. are the main symbionts in the three ticks and reside primarily in midgut, Malpighian tubules, and reproductive tissues, but their tissue distribution varies in association with species and sexes.\n <\/jats:p>\n \n IMPORTANCE<\/jats:bold>\n Tick-borne diseases constitute a major public health burden, as they are increasing in frequency and severity worldwide. The presence of symbionts helps ticks to metabolize nutrients, promotes fecundity, and influences pathogen infections. Increasing numbers of tick-borne pathogens have been identified in China; however, knowledge of native ticks, especially tick symbiosis, is limited. In this study, we analyze the distribution of\n Coxiella<\/jats:named-content>\n sp. and\n Rickettsia<\/jats:named-content>\n sp. in tissues of laboratory-reared\n Haemaphysalis longicornis<\/jats:named-content>\n and\n Rhipicephalus haemaphysaloides<\/jats:named-content>\n and field-collected\n Dermacentor silvarum<\/jats:named-content>\n . We found that the localization patterns of\n Coxiella<\/jats:named-content>\n sp. in three Chinese tick species were similar to those of other tick species. We also found a previously undefined intracellular localization of\n Rickettsia<\/jats:named-content>\n sp. in tick midgut and spermatids. In addition, we demonstrate that tissue tropisms of symbionts vary between species and sexes. Our findings provide new insights into the tissue localization of symbionts in native Chinese ticks and pave the way for further understanding of their functional capabilities and symbiotic interactions with ticks.\n <\/jats:p>","DOI":"10.1128\/aem.00029-18","type":"journal-article","created":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T12:35:35Z","timestamp":1520858135000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Tissue Localization and Variation of Major Symbionts in Haemaphysalis longicornis, Rhipicephalus haemaphysaloides, and Dermacentor silvarum in China"],"prefix":"10.1128","volume":"84","author":[{"given":"Mengfei","family":"Wang","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Tropical Diseases, Shanghai, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1871-8390","authenticated-orcid":false,"given":"Jianfeng","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Biology and Medical Sciences, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwei","family":"Zhong","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Tropical Diseases, Shanghai, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6794-2604","authenticated-orcid":false,"given":"Jingwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global vector control response 2017\u20132030","author":"WHO","year":"2017","unstructured":"WHO. 2017. 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