{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:28:15Z","timestamp":1790911695200,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and\n            <jats:italic>B. thailandensis<\/jats:italic>\n            is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this property for the rapid generation of marked deletion mutations by using PCR products. The method was employed to create 61 mutant strains. Several selectable elements were employed, including elements carrying\n            <jats:italic>loxP<\/jats:italic>\n            and\n            <jats:italic>FRT<\/jats:italic>\n            recombinase recognition sites to facilitate resistance marker excision. Chromosomal mutations could also be transferred readily between strains by transformation. The availability of simple procedures for creating defined chromosomal mutations and moving them between strains should facilitate genetic analysis of virulence and other traits of these two\n            <jats:italic>Burkholderia<\/jats:italic>\n            species.\n          <\/jats:p>","DOI":"10.1128\/aem.00030-08","type":"journal-article","created":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T02:37:03Z","timestamp":1204339023000},"page":"2985-2989","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Targeted Mutagenesis of\n            <i>Burkholderia thailandensis<\/i>\n            and\n            <i>Burkholderia pseudomallei<\/i>\n            through Natural Transformation of PCR Fragments"],"prefix":"10.1128","volume":"74","author":[{"given":"Metawee","family":"Thongdee","sequence":"first","affiliation":[{"name":"Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand"},{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry A.","family":"Gallagher","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Schell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tararaj","family":"Dharakul","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirirurg","family":"Songsivilai","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Manoil","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0616-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt715"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.12.4965-4968.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth765"},{"key":"e_1_3_2_6_2","first-page":"1064","volume":"74","year":"2007","unstructured":"Choi, K. H., T. Mima, Y. Casart, D. Rholl, A. Kumar, I. R. Beacham, and H. P. Schweizer. 2007. Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl. Environ. Microbiol.74:1064-1075.","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.11.e53"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403302101"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2036282100"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-6-174"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00369-4"},{"key":"e_1_3_2_13_2","first-page":"192","year":"2007","unstructured":"Logue, C., I. Peak, and I. Beacham. 2007. Genetic manipulation of Burkholderia pseudomallei, abstr. MOP012, p. 192. Abstr. 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Cold Spring Harbor Laboratory Press, New York, NY.","journal-title":"A short course in bacterial genetics."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000244046.31135.b3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01262-05"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-5-374"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)21015-2"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01558.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.10.4319-4321.1997"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7857-7862.2005"},{"key":"e_1_3_2_22_2","first-page":"756","volume":"30","year":"1999","unstructured":"Trakulsomboon, S., V. Vuddhakul, P. Tharavichitkul, N. Na-Gnam, Y. Suputtamongkol, and V. Thamlikitkul. 1999. Epidemiology of arabinose assimilation in Burkholderia pseudomallei isolated from patients and soil in Thailand. Southeast Asian J. Trop. Med. Public Health30:756-759.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_23_2","first-page":"81","volume":"10","year":"2007","unstructured":"Wiersinga, W. J., A. F. de Vos, R. de Beer, C. W. Wieland, J. J. Roelofs, D. E. Woods, and T. van der Poll. 2007. Inflammation patterns induced by different Burkholderia species in mice. Cell. Microbiol.10:81-87.","journal-title":"Cell. 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