{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:17:00Z","timestamp":1791296220998,"version":"4.1.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            strains are classified in at least three distinct phylogenetic lineages. There are correlations between lineage classification and source of bacterial isolation; e.g., human clinical and food isolates usually are classified in either lineage I or II. However, human clinical isolates are overrepresented in lineage I, while food isolates are overrepresented in lineage II. \u03c3\n            <jats:sup>B<\/jats:sup>\n            , a transcriptional regulator previously demonstrated to contribute to environmental stress responses and virulence in\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            lineage II strains, was hypothesized to provide differential abilities for\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            survival in various niches (e.g., food and human clinical niches). To determine if the contributions of \u03c3\n            <jats:sup>B<\/jats:sup>\n            to stress response and virulence differ across diverse\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            strains, \u0394\n            <jats:italic>sigB<\/jats:italic>\n            mutations were created in strains belonging to lineages I, II, IIIA, and IIIB. Paired parent and \u0394\n            <jats:italic>sigB<\/jats:italic>\n            mutant strains were tested for survival under acid and oxidative stress conditions, Caco-2 cell invasion efficiency, and virulence using the guinea pig listeriosis infection model. Parent and \u0394\n            <jats:italic>sigB<\/jats:italic>\n            mutant strain transcriptomes were compared using whole-genome expression microarrays. \u03c3\n            <jats:sup>B<\/jats:sup>\n            contributed to virulence in each strain. However, while \u03c3\n            <jats:sup>B<\/jats:sup>\n            contributed significantly to survival under acid and oxidative stress conditions and Caco-2 cell invasion in lineage I, II, and IIIB strains, the contributions of \u03c3\n            <jats:sup>B<\/jats:sup>\n            were not significant for these phenotypes in the lineage IIIA strain. A core set of 63 genes was positively regulated by \u03c3\n            <jats:sup>B<\/jats:sup>\n            in all four strains; different total numbers of genes were positively regulated by \u03c3\n            <jats:sup>B<\/jats:sup>\n            in the strains. Our results suggest that \u03c3\n            <jats:sup>B<\/jats:sup>\n            universally contributes to\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            virulence but specific \u03c3\n            <jats:sup>B<\/jats:sup>\n            -regulated stress response phenotypes vary among strains.\n          <\/jats:p>","DOI":"10.1128\/aem.00031-10","type":"journal-article","created":{"date-parts":[[2010,5,8]],"date-time":"2010-05-08T02:25:30Z","timestamp":1273285530000},"page":"4216-4232","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":92,"title":["<i>Listeria monocytogenes<\/i>\n            \u03c3\n            <sup>B<\/sup>\n            Has a Small Core Regulon and a Conserved Role in Virulence but Makes Differential Contributions to Stress Tolerance across a Diverse Collection of Strains"],"prefix":"10.1128","volume":"76","author":[{"given":"H. 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